ISSN:
1432-0983
Keywords:
Reciprocal recombination
;
Mitochondrial genome
;
Chondriome variability
;
Triticum aestivum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The mitochondrial genome of the selfed progeny of a plant regenerated from long-term somatic tissue culture displays specific structural rearrangements characterized by the appearance of novel restriction fragments. A mitochondrial DNA library was constructed from this selfed progeny in the SalI site of cosmid pHC79 and the novel fragments were subsequently studied. They were shown to arise from reciprocal recombination events involving DNA sequences present in the parental plant. The regions of recombination were sequenced and the nucleotide sequences were aligned with those of the presumptive parental fragments. We characterized an imperfect short repeated DNA sequence, 242 bp long, within which a 7-bb DNA repeat could act as a region of recombination. The use of PCR technology allowed us to show that these fragments were present in both parental plants and tissue cultures as low-abundance sequence arrangements.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351786
Permalink