ISSN:
1432-072X
Keywords:
5-Deazaflavin
;
Nucleotidyl-5-deazaflavin
;
Methanopterin
;
Oxygen sensitivity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Intracellular levels of F390 (AMP and GMP adducts of the 5-deazaflavin cofactor F420) in Methanobacterium thermoautotrophicum were analysed after gasing fermenter cultures with several consecutive cycles of substrate gas and gas mixtures containing 5% oxygen. No F390 was detected in growing cells, hydrogen starved cells and CO2 starved cells prior to O2 contamination. Also, no F390 was found in hydrogen depleted cells after O2 treatment. Exposure of exponentially growing cells and CO2 starved cells to oxygen lead to the formation of F390 species; the increase in the detected amount of F390 was coupled to a decrease of the F420 level. As soon as anaerobiosis was reestablished F390 cofactors were degraded and growth proceeded. Independent of the physiological condition of Methanobacterium thermoautotrophicum methanopterin was formed upon O2 exposure. After normal growth conditions were restored the level of detected methanopterin decreased again.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00407788
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