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  • Testcross  (2)
  • Medicago sativa L.  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Comparisons of alfalfa somaclonal and sexual derivatives from the same genetic source (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 263-266 
    Details
    ISSN: 1432-2242
    Keywords: Medicago sativa L. ; 2n gametes ; Tissue culture ; Chromosome doubling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tetraploid alfalfa (Medicago sativa L.) clones were derived from the same diploid genetic background by four different methods. A phenotypically superior clone was selected from each method and compared for herbage yield and fertility. The four methods and their best clones were: a) In vitro somatic chromosome doubling of one diploid hybrid (HG2-4x); b) selection within a two allele tetraploid synthetic population derived from HG2-4x (HAG); c) somaclonal variant selection from cell suspension culture of the diploid hybrid (NS1); and d) sexual polyploidization of a sibling hybrid (HXG). Clones HG2-4x, HAG, and NS1 were likely diallelic or monoallelic at all loci. Clone HXG was probably tetrallelic or triallelic at most loci. Experiments measured fertility, clonal herbage yield, and herbage yield of test cross progeny for each selected clone. Fertility rankings were HXG = HAG 〉 NS1 〉 HG2-4x. Clonal herbage yield rankings were HXG = HAG 〉 NS1 〉 HG2-4x. Test cross progeny herbage yield rankings varied depending on the tester, but, in general, HXG ≧ HAG ≧ NS1 HG2-4x. Overall the best clones from the sexual methods exceeded the best somaclonal variant which, in turn, was better than the chromosome doubled clone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317051
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  • 2
    Electronic Resource
    Electronic Resource
    Repeatability and heritability of divergent recombination frequencies in the Iowa Stiff Stalk Synthetic (Zea mays L.) (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 990-996 
    Details
    ISSN: 1432-2242
    Keywords: Crossing-over ; Testcross ; Corn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variability in recombination frequency was reported in the Iowa Stiff Stalk Synthetic. The objectives of the present research were to verify the differences in recombination frequency among individuals in the Iowa Stiff Stalk Synthetic maize population and to determine if the recombination frequency differences persisted among the S1 progeny. Testcrosses to measure male recombination frequency on three chromosomes (4, su1-c2; 5, a2-bt1-pr1; 9, sh1-bz1-wx1) were repeated for eight S0 individuals. Recombination frequencies were repeatably divergent among those individuals which were selected based on high or low recombination frequencies on specific chromosomes. Individuals which had been selected for long and short total map distances across the three chromosome regions produced repeatably divergent recombination frequencies only at the su1-c2 region. The recombination frequencies of the S1 lines, derived from the S0 individuals which had the most divergent recombination frequencies on a single chromosome, were significantly different. The broadsense heritability estimates derived from the regression of six S1 lines on six S0 individuals ranged from 0.69 to 0.20 for the five chromosome regions. We conclude that genetic differences for recombination frequency exist in this population and that modification by selection should be possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224103
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  • 3
    Electronic Resource
    Electronic Resource
    Variability of recombination frequencies in the Iowa Stiff Stalk Synthetic (Zea mays L.) (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 859-866 
    Details
    ISSN: 1432-2242
    Keywords: Crossing-over ; Testcross ; Quantitative inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variability in recombination frequency has been reported in several plant populations. The objectives of the present research were to establish the range in variability in recombination among genotypes in the important corn population Iowa Stiff Stalk Synthetic and to identify individual genotypes which produced increased or decreased recombination frequencies. Approximately 150 individual S0 plants were testcrossed to measure male recombination frequency on three chromosomes: 4, sul-c2; 5, a2-btl-pr1; and 9, sh1-bz1-wx1. Although the variance component for individuals accounted for only 20–33% of the total variation, highly significant variability among individuals was present at all chromosome regions. Thus the environmental effects did not prevent measurement of differences between S0 individuals. At each chromosome region, individual genotypes with recombination frequencies at least two standard deviations above or below the population mean were isolated. Reports in the literature suggest that the variability reported here for the BSSS population should be representative of that present in other corn breeding populations. Recombination frequencies were positively correlated between adjacent regions of chromosome 9 and also between adjacent regions of chromosome 5. Recombination frequencies were positively correlated between both regions on chromosome 5 with the su1-c2 region of chromosome 4. Negative correlations were observed between chromosome 9 recombination and recombination in each region of chromosomes 4 and 5. Thus rankings of S0 individual recombination frequencies were not consistent for all three chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212613
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