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  • 1
    Electronic Resource
    Electronic Resource
    Factors affecting the cellular expression of bacterial luciferase (1981)
    Springer
    Archives of microbiology 129 (1981), S. 67-71 
    Details
    ISSN: 1432-072X
    Keywords: Bioluminescence ; Marine bacteria ; Electron transport ; Low oxygen ; Long-chain aldehyde ; Luciferase expression quotient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The in vivo expression of cellular bacterial luciferase has been defined as the luciferase expression quotient, measured as the ratio of the bioluminescence intensity in vivo to the in vitro activity of luciferase in crude cell extracts. The expression is greater in the presence of inhibitors of the electron transport system such as cyanide and N-heptyl-4-hydroxyquinoline and also at lower oxygen tensions. The higher expression of the cellular luciferase under these conditions is postulated to be due to an increase in the intracellular levels of reduced coenzymes which enhance both the reduction of flavin and the reduction of fatty acid to aldehyde. Both FMNH2 and aldehyde are substrates in the light emitting reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417183
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of luminescence in Photobacterium phosphoreum: Na+ regulation of in vivo luminescence appearance (1986)
    Springer
    Archives of microbiology 145 (1986), S. 342-346 
    Details
    ISSN: 1432-072X
    Keywords: Bioluminescence ; Photobacterium phosphoreum ; Marine bacteria ; NaCl induction ; Autoinduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Photobacterium phosphoreum strain 496, growth and luminescence in a complex medium are optimal with 3% NaCl. However, in the same medium with 1% NaCl growth is similar, but the development of bioluminescence does not occur. In cells grown to mid or late-log phase in 1% NaCl, light emission can be triggered by the addition of NaCl, but the time required for its appearance is quite long, at least 30–45 min. The synthesis of m-RNA and protein are required for the development of luminescence, but the long time interval suggests that some intermediate steps are required. The time required is not less in conditioned 3% NaCl medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00470868
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  • 3
    Electronic Resource
    Electronic Resource
    Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria (1979)
    Springer
    Archives of microbiology 120 (1979), S. 87-91 
    Details
    ISSN: 1432-072X
    Keywords: Luminous bacteria ; Marine bacteria ; Induction ; Beneckea ; Photobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It has been previously demonstrated that luciferase synthesis in the luminous marine bacteria, Beneckea harveyi and Photobacterium fischeri is induced only when sufficient concentrations of metabolic products (autoinducers) of these bacteria accumulate in growth media. Thus, when cells are cultured in liquid medium there is a lag in luciferase synthesis. A quantitative bioassay for B. harveyi autoinducer was developed and it was shown that many marine bacteria produce a substance that mimics its action, but in different amounts, (20–130% of the activity produced by B. harveyi) depending on the species and strain. This is referred to as alloinduction. None of the bacteria tested produced detectable quantities of inducer for P. fischeri luciferase synthesis. These findings may have significance with respect to the ecology of B. harveyi and P. fischeri.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409093
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  • 4
    Electronic Resource
    Electronic Resource
    Mobilization of cloned luciferase genes into Vibrio harveyi luminescence mutants (1986)
    Springer
    Archives of microbiology 143 (1986), S. 325-329 
    Details
    ISSN: 1432-072X
    Keywords: Plasmid mobilization ; Bioluminescence ; Temperature conditional ; Complementation ; Marine bacteria ; Autoinduction ; Vibrio harveyi ; Luciferase genes ; Cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A recombinant plasmid which carried a 5 kb fragment of Vibrio harveyi DNA containing the luxA and luxB genes was mobilized from Escherichia coli into luminescence-deficient mutants of V. harveyi. The cloned genes complemented a temperature sensitive luciferase mutation, but failed to complement lesions in two different aldehyde deficient mutants. Expression of the cloned genes was not subject to autoinduction in either E. coli or in V. harveyi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412797
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