ISSN:
1432-072X
Keywords:
Immunoferritin labeling
;
Electron microscopy
;
Membrane vesicles
;
Nitrate reductase
;
Bacillus licheniformis
;
Klebsiella aerogenes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The indirect immunoferritin labeling method was used to localize the membrane-bound respiratory nitrate reductase in membrane vesicles and protoplasts or spheroplasts of Bacillus licheniformis and Klebsiella aerogenes, respectively. For a comparison of the labeling of the various vesicle preparations, which differed not only in size but also in the percentage of inside-out orientation, a quantification of the results was needed to circumvent the problem of non-specifically bound ferritin. From the results the sidedness of the nitrate reductase in the cytoplasmic membrane of the abovementioned bacteria was determined as being cytoplasmic in B. licheniformis and as transmembranous in K. aerogenes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414353
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