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  • Indole alkaloid (transport, accumulation)  (1)
  • Life and Medical Sciences  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Uptake and accumulation of ajmalicine into isolated vacuoles of cultured cells of Catharanthus roseus (L.) G. Don. and its conversion into serpentine (1991)
    Springer
    Planta 183 (1991), S. 170-177 
    Details
    ISSN: 1432-2048
    Keywords: Catharanthus ; Indole alkaloid (transport, accumulation) ; Peroxidase (vacuolar) ; Vacuole (alka-loid conversion)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated vacuoles from ajmalicine-producing cell suspensions of Catharanthus roseus accumulated the alkaloid ajmalicine. Dissipation of the transtonoplast pH gradient with nigericin abolished ajmalicine accumulation, whereas dissipation of the transtonoplast potential with valinomycin had no effect. Addition of Mg-ATP resulted in a higher ajmalicine accumulation. Serpentine produced by the cells was largely recovered in isolated vacuoles; in contrast, ajmalicine was lost. Ajmalicine was converted in vitro into serpentine by horseradish basic peroxidases (EC 1.11.1.7). In cultured cells there was a striking conformity between the time course of serpentine content and that of the activity of basic peroxidases. Ajmalicine was converted efficiently into serpentine by basic peroxidases extracted from vacuoles and by intact isolated vacuoles. The results are consistent with the hypothesis that ajmalicine accumulates by an ion-trap mechanism and that the accumulated ajmalicine is converted into serpentine inside the vacuoles. By the transformation of ajmalicine into the charged serpentine a trap is created to retain the alkaloids more efficiently in the vacuole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197785
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  • 2
    Electronic Resource
    Electronic Resource
    Transcriptional regulation of the major promoters of the human IGF-II gene (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 35 (1993), S. 391-393 
    Details
    ISSN: 1040-452X
    Keywords: PreproIGF-II ; P1 and P3 promoters ; C/EBP ; CCAAT/enhancer binding protein ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The human insulin-like growth factor-II (IGF-II) gene constitutes a complex transcriptional unit that is controlled by four different promoters preceding multiple 5′-untranslated exons. Expression of the IGF-II gene is development and tissue specific and yields a family of mRNAs that all encode preproIGF-II. We have analyzed the sequences and factors involved in the transcriptional activation of promoters P1 and P3. Detailed analysis revealed several characteristic elements and protein binding sequences. P1, which is expressed only in adult liver tissue, contains a region that can be specifically activated by the CCAAT/enhancer binding protein (C/EBP). P3 consists of a proximal promoter region that supports general transcription, whereas cell-specific elements are located upstream. © 1993 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080350412
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