Publication Date:
1995-05-05
Description:
Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, a hybrid immunoglobulin A-G heavy chain, a murine joining chain, and a rabbit secretory component, respectively. Successive sexual crosses between these plants and filial recombinants resulted in plants that expressed all four protein chains simultaneously. These chains were assembled into a functional, high molecular weight secretory immunoglobulin that recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble secretory antibodies, whereas two different cell types are required in mammals. Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy. Plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ma, J K -- Hiatt, A -- Hein, M -- Vine, N D -- Wang, F -- Stabila, P -- van Dolleweerd, C -- Mostov, K -- Lehner, T -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1995 May 5;268(5211):716-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, United Medical School, Guy's Hospital, London, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7732380" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Antibodies, Monoclonal/biosynthesis
;
Immunoglobulin A, Secretory/*biosynthesis/genetics
;
Immunoglobulin Fragments/*biosynthesis
;
Immunoglobulin G/biosynthesis
;
Immunoglobulin Heavy Chains/biosynthesis
;
Immunoglobulin Joining Region/biosynthesis
;
Immunoglobulin Light Chains/biosynthesis
;
Mice
;
Molecular Sequence Data
;
Plants, Genetically Modified/*immunology
;
Plants, Toxic
;
Rabbits
;
Secretory Component/biosynthesis
;
Tobacco
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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