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Immobilization ofβ-fructofuranosidase fromAureobasidium on DEAE-cellulose (1994)

Hayashi, Sachio ; Sasao, Sinji ; Takasaki, Yoshiyuki ; [et al.]

Springer

Journal of industrial microbiology and biotechnology 13 (1994), S. 103-105

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ISSN: 1476-5535

Keywords: Immobilization efficiency ; Continuous reaction ; Fructo-oligosaccharide ; Fructosyl-transferring enzyme

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary β-Fructofuranosidase, which produces fructo-oligosaccharides (1-kestose and nystose) from sucrose, was purified fromAureobasidium and immobilized on DEAE-cellulose at especially high efficiency (95%). The enzymatic profiles of the immobilized enzyme were almost identical to those of the native form except that the stability was slightly improved. The immobilized enzyme was stable during long-term continuous reaction for up to 360 h.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01584106

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