Publication Date:
2021-05-19
Description:
Shrimp production increasing rapidly in the world and in2013 the production reaches 4.2 MT. In Iran the shrimp
production is under development and estimated in 1393, 20 thousand tons produced. In this regards the important
subject is health and disease in shrimp farm. The white spot syndrome virus for second time appears in chabahar
and damage many farms. Because the aquaculture activity expand in the world in national, regional and
international scale, many emerge disease are endanger. In this regard the viral disease is very important and not
only decrease the production but also has a side effect in business and national economy. For control and
prevention the viral disease, the accurate methods such as PCR kit were developed. In this project the PCR
methods with sensitivity, specificity and efficacy was designed and used for detection viral disease. Many
viruses have several serotypes and in different area maybe new serotype induce the disease. For this reason, the
specific kit will be design. Three viruses consist of MBV, TSV and IHHNV are very pathogenic in shrimp farm
and need the specific PCR kit for detection them. In this project the MBV virus was identified and designs a new
primer with Oligo software and the primer amplified a part of DNA with 185 bp in the gel. The specificity and
sensitivity of primer were checked by IQ2000 Kit and the primer used for detection unknown samples.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Identification
;
Virus
;
Kit detection
;
New molecular methods
;
Aquatic
;
TSV
;
HPV
;
MBV
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
72pp.
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