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  • Hordeum (leaves)  (2)
  • Sucrose (translocation)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 191 (1993), S. 180-190 
    ISSN: 1432-2048
    Keywords: Amino acid concentration ; Hordeum (leaves) ; Metabolite compartmentation ; Subcellular volumes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Metabolite concentrations in subcellular compartments from mature barley (Hordeum vulgare L. cv. Apex) leaves after 9 h of illumination and 5 h of darkness were determined by nonaqueous fractionation and by the stereological evaluation of cellular and subcellular volumes from light and electron micrographs. Twenty one-day-old primary leaves of barley with a total leaf volume of 902 μL per mg chlorophyll were found to be composed of 27% epidermis, 42% mesophyll cells, 6% veins, 4.5% apoplast and 23% gas space. While in epidermal cells 99% of the volume was occupied by the vacuole, mesophyll cells with an average volume of 31.3 pL consisted of 23 pL (73%) vacuole, 4.6 pL (19%) chloroplasts, 2.06 pL (6,7%) cytosol (including smaller organelles and vesicles), 0.34 pL (1%) mitochondria and 107 fL (0.34%) nucleus. The differences between leaves harvested after 9 h of illumination and after 5 h of darkness were in the size of the stromal compartment and the starch grains therein. Subcellular metabolite concentrations were calculated from the compartmental volumes and metabolite contents of the compartments as determined by nonaqueous fractionation. The amino-acid concentrations in stroma and cytosol were rather similar after 9 h of illumination and 5 h of darkness. In contrast, the vacuolar amino-acid concentrations were about one order of magnitude lower than the stroma and cytosol values, and there was a slight increase in concentration after 5 h of darkness.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Amino acid ; Hordeum (leaves) ; Phloem transport ; Photosynthesis ; Spinacia (leaves) ; Sucrose (translocation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rates of CO2 fixation during the light period and the rates of CO2 release during the night period were measured using mature leaves from 39- to 49-d-old spinach (Spinacia oleracea L., US Hybrid 424; grown in 9 h light, 15 h darkness, daily) and mature leaves from 21-d-old barley (Hordeum vulgare L., cv. Apex; grown in 14 h light, 10 h darkness, daily). At certain times during the light and dark periods leaves were harvested for assay of their contents of soluble carbohydrates, starch, malate and the various amino acids. Evaluation of the results of these measurements shows that in spinach and barley leaves 46% and 26%, respectively, of the carbon assimilated during the light period is deposited in the leaves for export during the night period. Taking into account the carbon consumption in the source leaves by dark respiration, it is evaluated that rates of assimilate export during the light period from spinach and barley leaves [38 and 42 μatom C · (mg Chl)−1 · h−1] are reduced in the dark period to 16 μatom C · (mg Chl)−1 · h−1 in both species. The calculated C/N ratios of the photoassimilates exported during the dark period were 0.029 and 0.015 for spinach and barley leaves, respectively.
    Type of Medium: Electronic Resource
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