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  • 1
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    Multiple infections in shrimp Litopenaeus vannamei broodstock in commercial hatcheries in Khouzestan Province (2018)
    Details
    Publication Date: 2021-05-19
    Description: The present study was carried out to investigate the status of health and disease, and their impact on shrimp broodstock (Litopenaeus vannamei) in Khouzestan Province (Choeibde Area) in the south of Iran. From March 2012 until April 2013, about 140 broodstock and 5000 postlarvae (PLs) were collected from hatcheries and grow out farms. Clinical signs of samples were documented in take history forms and then the samples were transported to Iranian South Aquaculture Institute (Ahvaz). Bacterial and fungal studies were carried out on hemolymph, hepatopancreas and gill tissue and then the shrimps were preserved in Davidson Fixative for histopathology. A part of uropods was also preserved in ethyl alcohol for PCR study and detecting three viruses, WSSV, TSV and IMNV. A part of PLs was also preserved in ethyl alcohol for PCR and the remaining was preserved in Davidson Fixative for histopathology. The results showed that 5 bacteria consisting of Vibrio alginolyticus, V. proteolyticus, V. mimicus, A. hydrophila and Plesiomonas shigelloides and one fungi Aspergillus fumigatus were identified. The results of PCR exhibited that the broodstock was free of the three viruses and the PL was infected with WSSV. In histopathology some tissues showed the effects of Vibrio infection in different organs such as gill and midgut, and the infection of PL tissues showed the Cowdry type A inclusion bodies in WSSV. Two specific signs of abnormality were also exhibited in histopathology that we call them Pseudo inclusion and Reolike particles and we need to conduct a new study to clarify detailed information about these finding. This finding can be used for assessing the health of shrimp culture and prevention of disease in broodstock in Iran.
    Description: Published
    Keywords: Fish processing ; Litopenaeus vannamei ; Vibrio alginolyticus ; Plesiomonas shigelloides ; Aspergillus fumigatus ; Shrimp broodstock ; Health ; Disease ; Histopathology ; PCR ; Commercial
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.869-885
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  • 2
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    The Study on health situation of shrimp Litopenaus vannamei broodstoock production in earthen pond and comparing with fiberglass or cement tanks (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The investigation of health and diseas situation of shrimp broodstook (Litopenaeus vannamei) in Boushehr province in earthen pond and cancreat and fiberglas was carried out from May 2014 until July 2014 with collected 100 samples brood stook from earthen pond and 100 samples from earth and fiberglas thank.The clinical sign of samples documented in take history form and then the samples transport to Iran Shrimp Research Instituet in Bousheher. The bacterial and fungal studied was carried out with hemolymp, hepatopacreas and gill tissue and then the shrimp preserved in Davidson Fixative for histopathology. A part of uropoda also preserved in ethyl alcholo for PCR study and detecting viruses. The result showed 10 bacteria consist V.harveyii, V.parahaemolyticus, V.anguillarum, V.vulnificus, V.mimicus, V.damsela,V.nereis, plesiomonas shigelloides V.alginolyticus V.proteolyticus, in earthen pond and 5 bacteria consit ,V.alginolyticus V.proteolyticus ، V.parahaemolyticus ، V. damsela ،V.mimicus were identified in fiberglas thank. In this study 10 fungi consist Penecilium, Asp. Niger, Asp. Flavious, Asp. fomigatus, Acromonium, Ulocladium, Mucor, Cladosporium, Alternaria, Rhizopus, and 5 of them were identified in both broodstock from earthen pond and fiberglas thank. However 6 parasite consist of zoothamnium, vorticella, Acneta, Ephelota, Epistylis, Epistylis and Apastomom identified in earthen pond and three of them were identified in fiberglas thank. In histoplatholgy some tissue showed the effect of vibrio infecting in different organs as well in gill and midgut and the PCR examined were negative for all viruses. Regarding the produce healthy broodstock we need excuted the High Health procdure.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp broodstock ; Health ; Disaes ; Histopathology ; PCR ; Litopenaeus vannamei ; V.harveyii ; V.parahaemolyticus ; V.anguillarum ; V.vulnificus ; V.mimicus ; V.damsela ; V.nereis ; V.alginolyticus ; V.proteolyticus ; Earthen Pond ; Fiberglass ; Tank
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 87pp.
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  • 3
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    Assessing pathogenesis of the white spot syndrome virus (WSSV) in the whiteleged shrimp (Litopenaeus vannamei) (2021)
    In:  http://aquaticcommons.org/id/eprint/23622 | 18721 | 2018-07-12 19:36:06 | 23622 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: The pathogenesis of the white spot syndrome virus (WSSV) was studied experimentally with challenge exposure of one hundred post larvae Litopenaeus vannamei each aged 40 days. The shrimp L. vannamei larvae were first examined with Shrimp PC sub(a)R sub(e) Simplex detection kit and found to be free of WSSV. The Fenerropenaeus indicus tissues that showed the clinical signs and were PCR positive for the white spot disease (WSD) were used as the source of WSSV. The challenge exposure was accomplished by feeding minced tissue of the F. indicus to L. vannamei for 24 hours. Based on the results, the L. vannamei revealed the clinical sign of WSD after three days, the PCR was positive and all the shrimp specimens were died after ten days. The control group of the shrimp did not show any sign of WSD and their PCR was negative during the experiment.
    Keywords: Fisheries ; Health ; Pathogenesis ; Whiteleg shrimp ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-8
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  • 4
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    Identification of white spot syndrome virus in cultured Penaeus indicus by polymerase chain reaction (PCR) in I.R. IRAN (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25123 | 18721 | 2018-08-26 13:40:27 | 25123 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for Housekeeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
    Keywords: Health ; Iran ; White spot syndrome virus ; DNA ; Polymerase chain reaction ; WSD ; Viral ; Shrimp ; Samples ; Penaeus indicus ; Polymerase ; WSSV ; Virus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 39
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  • 5
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    Implementation biosecurity standard in shrimp specific pathogen free (SPF) research pilot study (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25552 | 18721 | 2018-10-05 17:30:01 | 25552 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Biosecurity means” all activities that decrease the introducing and expanding the pathogen in the shrimp farm” the main activity consisit physical, chemical and biological methods. Some factores has low risck and some of them have high risck level. The level of biosecurity in the shrimp farm and hatcheries is depends the equipmemt and expense that used in the program. For each situation in biosecurity, the scientist will be prepared the standard operation program (SOP) for each risck factor.In the Specific Pathogen Free program that conduted in Bousher, 10 SOP design for different risck factor in the banderga station and Persian Golf Centre for SPF production. The SOP, detecting the main pathogen in shrimp farm in shrimp, feed, water and any equipment in the project. The SOP also consider the disinfectant water with physical and chemival methods such as different net in different size, chemical such as chlorine and ozon and biological filter. During the project studied the methods exclude all pathogen from shrimp and feed and decreased the risck factors in program. The result showed if the culturist used these methods in the production, the safty of shrimp production increased and the sustainable shrimp culture will be available.
    Keywords: Health ; Iran ; Biosecurity ; Ciritical point ; Standard Operation Porogram ; Disaese ; SOP ; Implementation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 65
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  • 6
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    Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the algae Gracilaria corticata compared to shrimp fed without algae and exposed to white spot virus (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25807 | 18721 | 2018-10-13 10:13:41 | 25807 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: White spot disease (WSD) is one of the shrimp deadliest viral diseases that causes heavy losses on all shrimp of Penaeid family. Most invertebrates are lacking adaptive immune system and its defense is the innate immune system that is as cellular and humoral, but a like immune system against white spot virus in shrimp was been detected. In this research, control and prevention of white spot disease in shrimp using algae Gracilaria corticata, investigated. About 300 vannamei shrimp were divided to 4 groups and fed by normal pellet and algae extract in 14 days. At the end of the fourteenth day half of the shrimp were challenged with acute white spot virus. After the fourteenth day in the days 0, 3, 9, 18 and 25 sampling were done from the hemolymph of survived shrimps and survival and immune factors were evaluated. Based on results, in the challenge test, shrimps that fed with algae extract has a significant survival rate than shrimp fed with commercial diet. Increased the Immune Factors from day one to day 25 observed during the test. Greatest amount of Immune Factors THC, TPP, SOD, POD and PO in T1 group were observed in day 25 of tests. This situation was also true for group2 (T2), but its rate significantly was less than group 1(T1).
    Keywords: Aquaculture ; Health ; Iran ; Evaluation ; Immunity factors ; THC ; TPP ; PO ; SOD ; POD ; Shrimp ; Algae ; Gracilaria corticata ; White spot virus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 62
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  • 7
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    Detection and identification of carriers of white spot virus in wild shrimps and crabs in Persian Gulf and Oman Sea (Hormozgan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25180 | 18721 | 2018-09-03 17:09:40 | 25180 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: More than 20 viruses have been reported as pathogenic to shrimp.WSV has been found to be highly pathogenic not only to penaeid shrimps, but also to a wide range of hosts which include marine crabs , copepods, freshwater crabs and prawns. Main objective of this study was detection and identification of white spot virus from wild shrimp and crabs population on the coastal waters of Hormozgan Province.The samples were collected from three area seasonally include: coastal waters of Qeshm Island, Hengam Island and Jask. In this survey have been examined 1080 shrimp from each species of P.indicus, P.semisulcatus, P.merguiensis, Metapenaeus affinis and 1080 crabs (gill organs) by PCR and histopathological methods. Diagnostic kit for this survey have been prepared from Genesis Biotechnology CO.in Malaysia, so called "Single-Tube Nested PCR for WSSV". The analysis results revealed that all samples which examined from these area were free from WSV. Following PCR tests that were negative for all samples so no observed any damages of histology due to WSV on gills.
    Keywords: Health ; Iran ; Persian Gulf ; Oman Sea ; Hormozgan Province ; Qeshm Island ; Hengam Island ; Jask ; White Spot Disease ; Virus ; Penaeus ; PCR ; Histopathology ; Wild shrimps ; Crabs ; Pathogenic ; Marine ; Copepods ; Copepods ; P.indicus ; P.semisulcatus ; P.merguiensis ; Metapenaeus ; Genesis
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 43
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  • 8
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    Study on health and Immunity index of vaccinated Litopenaeus vannamei against white spot virus disease (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25541 | 18721 | 2018-10-05 16:29:38 | 25541 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In this study, the inhibitory effect of Radio-Vaccine against white spot disease on white shrimp (Litopenaeus vannamei) was studied. This vaccine was prepared by Nuclear Science and Technology Research Institute of Atomic Energy Organization of Iran. 14,000 L. vannamei postlarvae were used in treatments after adaptation. Eight treatments, four positive and two negative controls were considered in triplicate. Initially, three meals a day for a month in the hours of 8, 16 and 24 were feeding. Three times in day at 8, 16 and 24 clock were feeding in first mount. Waste food and fecal remaining in the tanks were removed. In the second and third months of treatment, feeding frequency increased to three and four times in day which is at 6, 18 and 24 were given concentrate feed and Artemia nauplii at 12 clocks. Vaccination was carried out by immersion. Vaccination was performed in two doses with an interval of 10 to 14 days. For biometric testing and evaluation of immune cells throughout the experiment, three samples were taken randomly from the tanks. To assess the health status of shrimp, indices of Total Hemocyte Count and Total Plasma Protein was determined. Phagocytic activity of hemocytes was evaluated to assess immune setuation of shrimps. The results of this study showed that the shrimp immune system, as in many reports, the effect of recombinant vaccines and passive safety in memory as "Alternative adaptive immune responses" after experimental infection of white spot virus (WSSV) in wild and farmed shrimp is confirmed, will respond to vaccination against white spot disease.
    Keywords: Aquaculture ; Health ; Iran ; Immunity index ; Litopenaeus vannamei ; White spot virus ; Disease ; White shrimp ; Feeding ; Artemia nauplii ; Samples ; Hemocyte ; Plasma Protein
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 48
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  • 9
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    Feasibility study of white spot syndrome virus vaccine with gamma radiation (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25549 | 18721 | 2018-10-05 17:27:04 | 25549 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The study of effect white spot syndrome virus(WSSV) attenuated vaccine that produced with gamma radiation on shrimp Litopenaeus vannamei carried out by imprinted 14000 PL from Kolahi hatchery in Hormozgan province. The shrimp divided to 3 groups as mentioned in table 2-1. The first group exposed to WSSV vaccine. The second group exposed to WSSV vaccine and then exposed to WSSV and the third did not exposed to vaccine and WSSV and named control positive. The experiment conducted 40 days and the shrimp cultured in standard trail. The result showed the shrimp exposed to vaccine and then exposed to WSSV has 76% and 61% higher survival rate after 35 days and 45 days respectively. The mortality rate also exhibited24% and 39% in both group respectively. The mean value of total hemocyte count and total protein plasma measuring with ANNOVA and LSD examination in group vaccinated and without vaccinate in exposing and injecting group showed no statistical differentiation (P〉0.05) while the total heamocyte count and total protein plasma showed statistical differentiation (P.〈0.05) between both groups. The result from histopathological examinations showed the cowdry type a inclusion bodies in all organ and tissue exhibited and the group vaccinated showed lower inclusion bodies with comparing without vaccinated.
    Keywords: Aquaculture ; Health ; Iran ; Litopenaus vannamei ; White spot syndrome virus vaccine ; White Spot Syndrome Virus ; Survival rate ; WSSV ; Feasibility ; Gamma ; Radiation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 106
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  • 10
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    Screening of pathological agent and recognize of disease in shrimp specific pathogen free (SPF) production (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25550 | 18721 | 2018-10-05 16:07:33 | 25550 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The production of specific pathogen free (SPF) of native shrimp is the first step for sustainable shrimp culture in Iran. This concept needs better understanding of health managmet and biosecurity in shrimp broodstock production for produc 25000MT in the five programe and development of Iran. In SPF center the main pathogen consist of viruses, bacteria and fungei that will be exclude the shrimp SPF.Theses pathogen divided to three categories, the first category consist of highly virlent pathogen such as white spot syndrome virus, Tura syndrome virus, Infection myonecrosis virus, infection hypodermal and hematopoietic virus. The second category are hepatopancratuc parvo like virus, and vibrio bacteria, Necrotizing hepatopnacratits, microsporidia and haplosporidia and the third category consist gregarines with light virlent.In the high level project that conducted in Iran between 2011 untill now about 8000 brodstoock collectd from farm culture in bousher province during September and October of 2012 from high health and Moluky stock. All shrimp tested for viruse, bacteria and fingai and the results showed the shrimp wrer free of viruse and bacterial pathogen. During the study one sample showed necrotizing hepatopancreatitis and we removed from the center.
    Keywords: Health ; Iran ; Survillence ; Disease ; Identification ; SPF ; Specific pathogen free ; Shrimp ; Shrimp culture ; Screening
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 76
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