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  • Haploid  (3)
  • embryogenesis  (3)
  • 1
    Electronic Resource
    Electronic Resource
    In vitro culture of isolated microspores and regeneration of plants in Brassica campestris (1992)
    Springer
    Plant cell reports 11 (1992), S. 234-237 
    Details
    ISSN: 1432-203X
    Keywords: Brassica campestris ; Microspore ; Embryogenesis ; Haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microspores were isolated from buds 2.0 – 2.9 mm in length and cultured in modified Lichter (1982) medium containing 17% sucrose, pH 6.2. After 48 h at 32°C, the incubation medium was replaced with NLN (Lichter 1982) medium containing 10% sucrose. Microspores were cultured at 24°C in darkness and embryos developed after three weeks. More than 1000 plants have thus far been regenerated. Genotypic differences were observed for microspore embryogenesis. The majority of the regenerants were haploid, however colchicine could be effectively used to achieve chromosome doubling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235072
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line (1995)
    Springer
    Plant cell reports 14 (1995), S. 580-584 
    Details
    ISSN: 1432-203X
    Keywords: Brassica rapa ; Microspore ; Embryogenesis ; Haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated microspore culture techniques are being widely used in Brassica breeding programs to generate haploid and doubled haploid plants. A number of factors influence regeneration response in vitro including genotype. In order to assess the effect of genotype on microspore embryogenesis in B. rapa L. var. oleifera, 17 cultivars and breeding lines were evaluated. Embryos developed from all but one genotype when using NLN medium with 17% sucrose, followed by a reduction in sucrose concentration to 10%, 48 h later. The number of embryos /100 buds differed between genotypes, ranging from 0 to 70. Further studies indicated that sucrose concentration and incubation time influenced embryogenesis. Selection studies carried out with an Agriculture and Agri-Food Canada breeding line have resulted in the identification of a highly embryogenic B. rapa line. This line produced thousands of microspore-derived embryos /100 buds and will be useful in mutant selection and gene transfer as well as biochemical and developmental studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231942
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  • 3
    Electronic Resource
    Electronic Resource
    Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 65-67 
    Details
    ISSN: 1432-2242
    Keywords: Brassica campestris ; Embryogenesis ; Haploid ; Microspore ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Culture of Brassica campestris anthers at 35°C for one or three days prior to culture at 25°C significantly stimulated the yield of microspore-derived embryos. More than 100 plants were regenerated from cultured embryos and haploids were identified amongst them. The haploid frequency was greater than 70% if all small-flowered sterile plants were considered to be haploid. The yield of microspore-derived plants in B. campestris is approaching the level where anther culture may be utilized as a practical breeding tool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285191
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. (1991)
    Springer
    Euphytica 58 (1991), S. 51-55 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; donor plant age ; embryogenesis ; haploid ; microspore culture ; microspore stage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effect of age of donor plants and age of inflorescence on embryogenesis in microspore culture of B. napus was examined. Microspores isolated from buds of older plants had a higher embryo yield than those of younger ones. The effect of the age of inflorescence showed a different pattern. In older plants, a higher embryogenesis response was observed in microspores isolated from buds of new inflorescences, while in young plants, microspores isolated from buds of old inflorescences showed high embryo yield. These different responses were considered to be attributable to a difference in the developmental stage of pollen at the time of microspore isolation. Our results indicated that microspores collected from older inflorescences and older plants have sufficient embryogenic potential when the optimum developmental stage of pollen was used. Frequency of embryo to plant conversion was influenced by the size of embryos subcultured, but not by donor plant age or the age of the inflorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035339
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  • 5
    Electronic Resource
    Electronic Resource
    Dry artificial seeds and desiccation tolerance induction in microspore-derived embryos of broccoli (1993)
    Springer
    Plant cell, tissue and organ culture 35 (1993), S. 121-129 
    Details
    ISSN: 1573-5044
    Keywords: abscisic acid ; Brassica oleracea ; embryogenesis ; microspore culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Desiccation tolerance of broccoli microspore-derived embryos was induced by exogenous application of abscisic acid (ABA). Embryos, which were desiccated to about 10% water content, were estimated for viability after rehydration. Survival was dependent on the ABA concentration and the development stage of embryo, but not on the length of exposure period to ABA or genotype. Cotyledonary stage embryos acquired the highest desiccation tolerance when treated with 1×10-4M ABA. Under this condition, on average 27–48% of the desiccated embryos could convert into plants. Embryos treated with 1×10-6M ABA or no ABA or earlier development-staged embryos, such as globular and heart stages, lost viability after desiccation. A one day exposure to ABA had the similar effect on the induction of desiccation tolerance as a 7-day treatment. The dried embryos maintained their ability of plant conversion after three months of storage under room conditions. The plants derived from the desiccated embryos were not different in the morphology or ploidy level from those from non-desiccated ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032961
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  • 6
    Electronic Resource
    Electronic Resource
    Production of haploids via anther culture in Brassica oleracea var. Italica (1983)
    Springer
    Euphytica 32 (1983), S. 151-159 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea var. italica ; broccoli ; anther culture ; embryo culture ; haploid ; embryogenesis ; organogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Culture of broccoli (cv. Green Mountain) anthers at elevated temperatures (e.g. 35°C for two days) prior to maintenance at 25°C stimulated microspore embryogenesis. Embryo yields were also increased by subjecting excised inflorescences to a short term, high temperature shock (i.e. 45°C for one hour followed by 40°C for three hours) prior to anther plating and by a ten-fold increase in the auxin level of the anther culture medium. Plants were regenerated from microspore-derived embryos either directly through embryo culture or through the induction of organogenesis in explants of embryos which failed to develop on embryo culture medium. Approximately 50% of the regenerates were haploids with the remainder considered to be spontaneously-doubled haploids. Haploids were cloned and maintained as axial bud cultures for three years without the occurrence of ploidy changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036875
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