ISSN:
1432-072X
Keywords:
Desulfovibrio vulgaris
;
Dissimilatory sulfate reduction
;
Growth yields
;
Chemostat cultures
;
Pyruvate metabolism
;
ATP synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Desulfovibrio vulgaris (strain Marburg) was grown on H2 and sulfate as sole energy source in a chemostat limited by the sulfate supply. The biomass concentration and the sulfate concentration in the culture were determined as a function of the dilution rate. From the data a K S (saturation constant) for sulfate of 10 μM, a μmax of 0.23 h−1, and a $${\text{Y}}_{{\text{SO}}_{\text{4}} ^{2 - } }^{{\text{max}}}$$ of 13 g/mol were calculated. The organism was also grown in chemostat culture on H2 and sulfite, H2 and thiosulfate, and pyruvate (without sulfate). $${\text{Y}}_{{\text{SO}}_3 ^{2 - } }^{{\text{max}}}$$ was found to be 35 g/mol, $${\text{Y}}_{{\text{S}}_{\text{2}} {\text{O}}_{\text{3}} ^{2 - } }^{{\text{max}}}$$ 36 g/mol, and Y pyr max 10 g/mol. The growth yields are discussed with respect to ATP gains in dissimilatory sulfate reduction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414550
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