Publication Date:
2014-06-21
Description:
How botulinum neurotoxins (BoNTs) cross the host intestinal epithelial barrier in foodborne botulism is poorly understood. Here, we present the crystal structure of a clostridial hemagglutinin (HA) complex of serotype BoNT/A bound to the cell adhesion protein E-cadherin at 2.4 angstroms. The HA complex recognizes E-cadherin with high specificity involving extensive intermolecular interactions and also binds to carbohydrates on the cell surface. Binding of the HA complex sequesters E-cadherin in the monomeric state, compromising the E-cadherin-mediated intercellular barrier and facilitating paracellular absorption of BoNT/A. We reconstituted the complete 14-subunit BoNT/A complex using recombinantly produced components and demonstrated that abolishing either E-cadherin- or carbohydrate-binding of the HA complex drastically reduces oral toxicity of BoNT/A complex in vivo. Together, these studies establish the molecular mechanism of how HAs contribute to the oral toxicity of BoNT/A.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164303/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164303/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lee, Kwangkook -- Zhong, Xiaofen -- Gu, Shenyan -- Kruel, Anna Magdalena -- Dorner, Martin B -- Perry, Kay -- Rummel, Andreas -- Dong, Min -- Jin, Rongsheng -- 1R01NS080833-01/NS/NINDS NIH HHS/ -- 1R56AI097834-01/AI/NIAID NIH HHS/ -- 8P51OD011103-51/OD/NIH HHS/ -- P30 NS076411/NS/NINDS NIH HHS/ -- P41 GM103403/GM/NIGMS NIH HHS/ -- R01 AI091823/AI/NIAID NIH HHS/ -- R01 NS080833/NS/NINDS NIH HHS/ -- R01AI091823/AI/NIAID NIH HHS/ -- R56 AI097834/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2014 Jun 20;344(6190):1405-10. doi: 10.1126/science.1253823.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA. ; Department of Microbiology and Immunobiology, Harvard Medical School, Division of Neuroscience, New England Primate Research Center, Southborough, MA 01772, USA. ; Institut fur Toxikologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. ; Centre for Biological Threats and Special Pathogens-Biological Toxins (ZBS3), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany. ; Northeastern Collaborative Access Team (NE-CAT) and Department of Chemistry and Chemical Biology, Cornell University, Building 436E, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA. ; Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA. r.jin@uci.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24948737" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Botulinum Toxins, Type A/*chemistry/genetics
;
Cadherins/*chemistry/genetics
;
Crystallography, X-Ray
;
Gene Knockdown Techniques
;
HT29 Cells
;
Hemagglutinins/*chemistry/genetics
;
Humans
;
Mice
;
Protein Structure, Secondary
;
Recombinant Proteins/chemistry
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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