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  • 1
    Electronic Resource
    Electronic Resource
    Multicolour whole-mount in situ hybridization to Drosophila embryos (1996)
    Springer
    Development genes and evolution 206 (1996), S. 292-295 
    Details
    ISSN: 1432-041X
    Keywords: Key words Biotin-UTP ; Alkaline phosphatase substrates ; Fast Red ; Fast Blue B ; Magenta-phos/INT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050055
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