ISSN:
1432-1432
Keywords:
Lysozyme
;
Enzyme immunoassay
;
Antigenic cross-reactivity
;
ELISA
;
Locust
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We used an indirect enzyme-linked immunosorbent assay (ELISA) for measuring the immunological cross-reactivities between bird lysozymes and a lysozyme isolated from the blood of the insectLocusta migratoria. The degrees of cross-reactivity among five avian lysozymes measured by ELISA agreed approximately with those observed in earlier work using microcomplement fixation tests. This latter technique is not suitable for detecting immunological cross-reactivity between proteins that differ in sequence by more than 30%–40%. In contrast, ELISA is able to detect distant relationships between antigens such as lysozymes that differ in sequence by as much as 60%. It seems likely that the use of ELISA procedures will extend the range of homologous proteins that can be compared by immunochemical means.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02100623
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