ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Preservatives-antioxidants
;
Eluent pH
;
Retention and separation factors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary In this paper the dependence of eluent pH on the efficiency of separation in RP-HPLC is described. Preservatives-antioxidants, esters ofp-hydroxybenzoic acid (methyl, ethyl, propyl and butyl parabens), sorbic acid, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), propyl gallate (PG) and L-ascorbic acid (Vitamine C) were investigated by reversed-phase high-performance liquid chromatography, utilising isocratic elution with UV detection. The mobile phase composition (methanol-water v/v containing 1% acetic acid), was varied in order to obtain the best separation. For all substances analysed the pH, pKa and the fitted pKa values were calculated in different mixtures of mobile phases using the graphical method of Charlot and Trémillon [1]. The retention factors (k) and the separation factors (α) were also calculated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02315132
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