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  • 1
    Electronic Resource
    Electronic Resource
    Lateral diffusion of erythrocyte phospholipids in model membranes comparison between inner and outer leaflet components (1990)
    Springer
    European biophysics journal 18 (1990), S. 33-41 
    Details
    ISSN: 1432-1017
    Keywords: Phospholipid asymmetry ; Lateral diffusion ; Photobleaching ; Spin-labels ; ESR ; Cholesterol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The physical properties of lipid bilayers with a similar composition to the outer and inner leaflets of the human erythrocyte membrane have been examined in protein-free model systems. The outer leaflet (OL) was represented by a phospholipid mixture containing phosphatidylcholine and sphingomyelin extracted from human erythrocytes, while a mixture of phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine represented the inner leaflet (IL). The ratio of cholesterol to phospholipid was varied in both mixtures. The lateral diffusion coefficient of fluorescent phospholipids diluted in such lipid mixtures was determined by the modulated fringe pattern photobleaching technique. Contrast curves with a single exponential decay, indicative of homogeneous samples, were obtained only for temperatures above 15 °C and for a cholesterol to phospholipid molar ratio below 0.8. The rate of lateral diffusion was approximately five times faster in IL than in OL multilayers, in agreement with former results obtained in human erythrocytes (Morrot et al. 1986). Varying the cholesterol to phospholipid ratio from 0 to 0.8 (mol/mol) enabled us to decrease the diffusion constant by only a factor of approximately 2 for both IL and OL mixtures. The order parameter of a spin-labeled phospholipid was determined in the different systems and found to be systematically smaller in IL mixtures than in OL mixtures. The present study indicates that the difference in lipid diffusivity of the two erythrocyte leaflets may be accounted for solely by a difference in phospholipid composition, and may be independent of cholesterol and protein asymmetry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185418
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of antiaggregant molecule ajoene with membranes (1989)
    Springer
    European biophysics journal 17 (1989), S. 211-216 
    Details
    ISSN: 1432-1017
    Keywords: Ajoene ; drug-membrane interaction ; NMR ; ESR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The structure of ajoene, a molecule extracted from garlic, has been studied by 1H-NMR and its interaction with model membranes by 1H-, 2H-, 31-P-NMR and ESR experiments. This study clearly shows that the ajoene molecule is located deep in the layer and is close to the interlayer medium. Moreover while NMR experiments show that the membrane structure is only slightly affected by the presence of ajoene, ESR experiments reveal significant modifications in phospholipid dynamics. This interaction, observed before with the phenothiazine derivative, promazine, results in an increase of the membrane fluidity in its hydrophobic part and could be related to clinical properties of ajoene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284727
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    Paper (German National Licenses)
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