Publication Date:
2007-05-19
Description:
Significant fractions of eukaryotic genomes give rise to RNA, much of which is unannotated and has reduced protein-coding potential. The genomic origins and the associations of human nuclear and cytosolic polyadenylated RNAs longer than 200 nucleotides (nt) and whole-cell RNAs less than 200 nt were investigated in this genome-wide study. Subcellular addresses for nucleotides present in detected RNAs were assigned, and their potential processing into short RNAs was investigated. Taken together, these observations suggest a novel role for some unannotated RNAs as primary transcripts for the production of short RNAs. Three potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes. These data support a highly interleaved organization of the human transcriptome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kapranov, Philipp -- Cheng, Jill -- Dike, Sujit -- Nix, David A -- Duttagupta, Radharani -- Willingham, Aarron T -- Stadler, Peter F -- Hertel, Jana -- Hackermuller, Jorg -- Hofacker, Ivo L -- Bell, Ian -- Cheung, Evelyn -- Drenkow, Jorg -- Dumais, Erica -- Patel, Sandeep -- Helt, Gregg -- Ganesh, Madhavan -- Ghosh, Srinka -- Piccolboni, Antonio -- Sementchenko, Victor -- Tammana, Hari -- Gingeras, Thomas R -- N01-CO-12400/CO/NCI NIH HHS/ -- U01HG003147/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2007 Jun 8;316(5830):1484-8. Epub 2007 May 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Affymetrix Laboratory, Affymetrix, Inc., 3420 Central Expressway, Santa Clara, CA, 95051, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17510325" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line, Tumor
;
Cell Nucleus/metabolism
;
Cytosol/metabolism
;
Exons
;
Gene Expression
;
Genome
;
*Genome, Human
;
HeLa Cells
;
Humans
;
Mice
;
Promoter Regions, Genetic
;
RNA/*genetics/metabolism
;
RNA Precursors/*genetics/metabolism
;
RNA, Messenger/*genetics/*metabolism
;
Synteny
;
Terminator Regions, Genetic
;
*Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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