ISSN:
1432-072X
Keywords:
Cyanobacteria
;
Synechocystis PCC 6803
;
Phycobilisome structure
;
Pigment mutants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mutants affected in their pigment content and in the structure of their phycobilosomes (PBS) were isolated in the cyanobacterium Synechocystis PCC 6803 by enriching a population with the inhibitor p-hydroxymercuribenzoate. Three of these mutants, PMB 2, PMB 10 and PMB 11, with original phenotypes, are described. Applying several criteria of analysis (77K absorption and fluorescence, protein electrophoretic patterns, electron microscopy), it was possible to assign the component polypeptides to each substructure of the phycobilisome. The model structure obtained fits with those described in other species PMB 10 and PMB 11, completely lacking PC, are the first source of pure PBS cores available, in which no contamination by residual PC can be feared, and are thus particularly interesting for further biochemical studies. The capacity of genetic transformation of Synechocystis PCC 6803 by chromosomal DNA makes this system very convenient for the analysis of the regulation of synthesis of the PBS constituents.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00402349
Permalink
