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  • 1
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    The importance of voucher specimens: misidentification or previously unknown mtDNA diversity in Phalacrocorax capillatus (Aves: Phalacrocoracidae)? (2023)
    Informa UK Limited
    In:  Mitochondrial DNA Part B vol. 8 no. 11, pp. 1158-1160
    Details
    Publication Date: 2024-01-12
    Description: A recently published complete mitochondrial genome of Japanese or Temminck\xe2\x80\x99s cormorant \n(Phalacrocorax capillatus) was the first of this species (GenBank accession number LC714913). \nComparison of COI sequences shows that this mitogenome clustered with great cormorant \n(Phalacrocorax carbo) rather than with its sister taxon P. capillatus. This suggests that the mitogenome \nwas either a misidentified P. carbo or represents previously unknown intraspecific diversity in P. capillatus overlapping with that of P. carbo. Unfortunately, no voucher specimen was retained so it remains \nimpossible to distinguish between these alternatives. We suggest that great restraint should be exercised using this mitogenome as a reference for P. capillatus. We reiterate previous pleas to retain voucher specimens for mitogenome sequences to enable re-analysis of the identity of the material.
    Keywords: Cormorants ; incorrect identification ; mitogenome
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 2
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    The published complete mitochondrial genome of Spotted Greenshank (Tringa guttifer) is a chimera with DNA from Red-necked Stint (Calidris ruficollis) (Aves: Charadriiformes) (2023)
    In:  Mitochondrial DNA Part B vol. 8 no. 11, pp. 1273-1275
    Details
    Publication Date: 2024-04-22
    Description: A recently published complete mitochondrial genome of Spotted Greenshank (Tringa guttifer) was the first DNA sequence of this species (GenBank accession number MK905885, RefSeq number NC_044665; Liu et al. 2019, The complete mitochondrial genome of the Spotted Greenshank Tringa guttifer (Charadriiforemes [sic]: Charadriidae), Mitochondrial DNA Part B. 4:2353–2354). Here we show that this mitogenome is actually a chimera containing DNA fragments of both a Tringa sandpiper (presumably T. guttifer) and the Red-necked Stint (Calidris ruficollis). This mitogenome has been re-used in at least three phylogenies. The error is documented to avoid the perpetuation of erroneous sequence information in the literature.
    Keywords: Chimerism ; laboratory ; errors ; mitogenome ; sequence artifacts ; shorebirds
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 3
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    Complete mitochondrial genome MK992912 of Great Knot (Calidris tenuirostris) is a chimera with DNA from Pacific Golden Plover Pluvialis fulva (Aves: Charadriiformes) (2024)
    In:  Mitochondrial DNA Part B vol. 9 no. 4, pp. 532-535
    Details
    Publication Date: 2024-04-28
    Description: A complete mitochondrial genome of Great Knot (Calidris tenuirostris), MK992912, was published by He and colleagues in 2020. Here we show that this mitogenome is actually a chimera containing DNA fragments of both C. tenuirostris (15,567 bp, 92.8%) and Pacific Golden Plover (Pluvialis fulva, 1208 bp, 7.2%). Detecting such errors is possible before publication if each sequenced fragment is separately analyzed phylogenetically before assembling the fragments into a single mitogenome. This mitogenome has been re-used in at least four phylogenies. The error is documented to avoid the perpetuation of erroneous sequence information in the literature.
    Keywords: Chimerism ; laboratory ; errors ; mitogenome ; sequence artifacts ; shorebirds
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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