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  • 1
    Electronic Resource
    Electronic Resource
    Application of the Laplacian filter to high-resolution enhancement of SEM images (1984)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 331-340 
    Details
    ISSN: 0741-0581
    Keywords: Digital image processing ; Laplacin filter ; Scanning electron microscopy ; High-resolution ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Certain digital image-processing methods, which are useful for nonperiodic structural images, have been applied to high-resolution SEM images for the improvement of resolution. Samples utilized in the present study consisted of magnetic tape coated with gold, T4 phage coated with gold-palladium, and uncoated specimens of Prolamellar body (PLB) in Cucurbita moschata. These images were blurred and otherwise disturbed by electronic noise, though the images were taken at the limit of efficiency of intrinsic instrument. The major image-processing tool was the Laplacian filter, which subtracts the Laplacian from the original image. Noise, which is a serious problem in digital processing of high-resolution SEM images, was suppressed by the nonlinear type smoothing method. Also, the noise was evaluated by an autocorrelation function and a power spectrum of the image. By using these methods of “deblurring” and noise removal, we achieved better resolution, and structural details of our biological specimens were revealed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060010403
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  • 2
    Electronic Resource
    Electronic Resource
    Serial section reconstruction using a computer graphics system: Applications to intracellular structures in yeast cells and to the periodontal structure of dogs' teeth (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 11 (1989), S. 16-26 
    Details
    ISSN: 0741-0581
    Keywords: 3-D reconstruction ; Computer graphics ; Serial sections ; Yeast target cell ; Tooth movement ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A computer graphics system for reconstruction from serial section micrographs was applied to intracellular details of a yeast target cell (Saccharomyces cerevisiae cell) induced by the α factor mating pheromone and was also applied to a periodontal structure of a dog tooth moved orthodontically. In the former, intracellular organelles and a distribution of vesicles could be clearly observed through the cell membrane using the transparent display method in which the smoothing of the reconstructed outer cell membrane surface by computer processing was applied to the transparent display. In the latter case, by cutting through a reconstructed dog tooth and its periodontal tissues, labiolingual and mesiodistal cut surfaces of the tooth and of adjacent alveolar bone could be observed with fine details (232 sections were used).
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060110104
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  • 3
    Electronic Resource
    Electronic Resource
    Digital image processing for nonperiodic objects and an on-line image processing system for high resolution electron microscopy (1986)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 4 (1986), S. 55-62 
    Details
    ISSN: 0741-0581
    Keywords: Autocorrelation ; Noise removal ; On-line image processing ; Diffractogram ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A digital image processing method for noise removal and image enhancement in nonperiodic structural images is described. The method for noise removal uses a reversible transform between an image and image autocorrelation function. The Laplacian filter is then employed for image enhancement. Furthermore, an on-line image processing system for highresolution TEM is presented.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060040106
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  • 4
    Electronic Resource
    Electronic Resource
    Three-dimensional reconstruction from serial section images by computer graphics (1986)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 3 (1986), S. 401-406 
    Details
    ISSN: 0741-0581
    Keywords: 3-D Reconstruction ; Serial thin sections ; Computer graphics ; Colonic adenoma ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A method is described that allows a three-dimensional object to be reconstructed from micrographs of serial sections by means of computer graphics. The reconstructed object, which can be rotated three-dimensionally, is displayed on a colour visual display unit, and the object is shaded in order to provide an illusion of a three-dimensional structure. Moreover, the technique makes it possible to observe an inner structure as seen through an outer one.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060030404
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  • 5
    Electronic Resource
    Electronic Resource
    The conversion of a field-emission scanning electron microscope to a high-resolution, high-performance scanning transmission electron microscope, while maintaining original functions (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 6 (1987), S. 15-30 
    Details
    ISSN: 0741-0581
    Keywords: STEM ; On-line computer system ; Digital image processing ; Unstained biological specimen ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Recently, the reliability of field-emission electron guns has increased. In addition, the cost of computer systems for on-line processing has dropped. Hence, we should now consider the use of scanning transmission electron microscopy (STEM) for routine work, especially, in the field of biology where one may expect to utilize digital image processing techniques.An STEM has been constructed, without disturbing the original functions, by converting a commercial scanning electron microscope equipped with a fieldemission gun. The STEM is generally operated at accelerating voltage 30 kV, focal length 7.5 mm, and beam current 1-2 × 10-10 A. Several improvements have been incorporated for removing the effects of vibration, contamination, and stray magnetic fields. Also, an adjustable detector aperture was utilized. The modified instrument was connected to an on-line digital image processing system for utilizing the information obtained from STEM images. The advantages of the modified system were studied from various viewpoints.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060060104
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