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  • 1
    Electronic Resource
    Electronic Resource
    Chromatin structure variation in successful and unsuccessful arbuscular mycorrhizas of pea (1993)
    Springer
    Protoplasma 175 (1993), S. 1-8 
    Details
    ISSN: 1615-6102
    Keywords: Chromatin structure ; DAPI ; DNase I ; Flow cytometry ; Pea arbuscular mycorrhizas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lincoln and Frisson varieties of endomycorrhiza-forming pea plants and isogenic mycorrhiza-resistant Frisson mutant (P2) plants were inoculated withGlomus mosseae. Nuclei released from inoculated and non-inoculated (control) roots were analysed for chromatin structure and activity using flow cytometric techniques. Chromatin accessibility to the specific DNA fluorochrome DAPI at saturating and non-saturating concentrations was measured. DNA fluorescence of nuclei of mycorrhizal Lincoln and wild genotype Frisson plants was significantly increased, compared to the controls, at saturating and, more strongly, at non-saturating DAPI concentrations. In contrast, the nuclei of inoculated P2 mutant roots showed a much lower increase in fluorescence, compared to uninoculated controls. Nuclei released from mycorrhiza-infected Lincoln roots were more sensitive to DNase I than those of uninfected ones. These results indicate a dramatic increase in that portion of the genome which can be transcribed in response to AM infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403279
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  • 2
    Electronic Resource
    Electronic Resource
    Studies of VA mycorrhizaein vitro: mycorrhizal synthesis of axenically propagated wild cherry (Prunus avium L.) plants (1983)
    Springer
    Plant and soil 71 (1983), S. 217-221 
    Details
    ISSN: 1573-5036
    Keywords: Cherry rootstocks ; Gigaspora margarita ; In vitro mycorrhizae ; Micropropagation ; Prunus avium L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An increasing number of woody plant species can be axenically propagated using plant tissue culture methods. These techniques produce high quality healthy plants but transplant problems often arise due to their being susceptible to different environmental stresses. Mycorrhizae can play a vital role in plant nutrition and survival and their introduction during the production of axenically propagated plants could contribute to the success of this technique. Using a simple method for axenic establishment and direct observation of VA mycorrhizal cultures,in vitro mycorrhizal synthesis of a woody plant species has been obtained for the first time using axenically propagated plants ofPrunus avium L. (wild cherry) and the VA fungusGigaspora margarita.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182657
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    Paper (German National Licenses)
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