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  • 1
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Footprinting in vivo ; Gene expression (transient) ; Light regulation (UV-B photoreceptor, blue-light photoreceptor) ; Petroselinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo footprinting in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and bluelight-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Computational optimization and applications 3 (1994), S. 7-26 
    ISSN: 1573-2894
    Keywords: network programming ; assignment ; integer programming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This manuscript presents a new heuristic algorithm to find near optimal integer solutions for the singly constrained assignment problem. The method is based on Lagrangian duality theory and involves solving a series of pure assignment problems. The software implementation of this heuristic, ASSIGN+1, successfully solved problems having one-half million binary variables (assignment arcs) in less than 17 minutes of wall clock time on a Sequent Symmetry S81 using a single processor. In computational comparisons with MPSX and OSL on an IBM 3081D, the specialized software was from 100 to 1,000 times faster. In computational comparisons with the specialized code of Mazzola and Neebe, we found that ASSIGN+1 was 40 times faster. In computational comparisons with our best alternating path specialized code, we found that ASSIGN+1 was more than three times faster than that code. This new software proved to be very robust as well as fast. The robustness is due to an elaborate scheme used to update the Lagrangean multipliers and the speed is due to the fine code used to solve the pure assignment problems. We also present a modification of the algorithm for the case in which the number of jobs exceeds the number of men along with an empirical analysis of the modified software.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Computational optimization and applications 8 (1997), S. 287-299 
    ISSN: 1573-2894
    Keywords: assignment problem ; integer programming ; Lagrangean relaxation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This manuscript presents a truncated branch-and-bound algorithm toobtain a near optimal solution for the constrained assignment problemin which there are only a few side constraints. At each node of thebranch-and-bound tree a lower bound is obtained by solving a singlyconstrained assignment problem. If needed, Lagrangean relaxationtheory is applied in an attempt to improve this lower bound. Aspecialized branching rule is developed which exploits therequirement that every man be assigned to some job. A softwareimplementation of the algorithm has been tested on problems with fiveside constraints and up to 75,000 binary variables. Solutionsguaranteed to be within 10% of an optimum were obtained for these75,000 variable problems in from two to twenty minutes of CPU time ona Dec Alpha workstation. The behavior of the algorithm for variousproblem characteristics is also studied. This includes the tightnessof the side constraints, the stopping criteria, and the effect whenthe problems are unbalanced having more jobs than men.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2019-07-13
    Description: No abstract available
    Keywords: Fluid Mechanics and Thermodynamics
    Type: 9th International Planetary Probe Workshop (IPPW-9); Jun 18, 2012 - Jun 22, 2012; Toulouse; France
    Format: text
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  • 5
    Publication Date: 2019-07-13
    Description: Aerocapture is a very useful capability for NASA that can be used across a wide range of planetary mission sizes and destinations. A substantial mass advantage may be realized through aerocapture maneuver implementation. The mass advantage is enabling for certain outer planet mission profiles. Aerocapture technology provides corollary benefits to the related applications of atmospheric entry and precision landing on worlds with atmospheres through aero/aerothermodynamic model validation, hypersonic guided flight, tps materials, and performance model validation. The ST9 Aerocapture flight validation will be sufficient to immediately infuse aerocapture technology into future NASA science missions. The advanced technologies being flight validated will enable the system level goal of performing an aerocapture maneuver. The advanced technologies include: The GN&C System, TPS materials, plus Advanced recession and heat flux sensors.
    Keywords: Fluid Mechanics and Thermodynamics
    Type: M09-0147 , 42nd AIAA/ASME/SAE/ASEE Joint Propulsion Conference and Exhibit; Jul 10, 2006 - Jul 12, 2006; Sacramento, CA; United States
    Format: application/pdf
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