ISSN:
1432-072X
Keywords:
Cytoplasmic fibrils
;
Treponemes
;
Treponema refringens
;
Fine structure
;
Cell lysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The cytoplasmic fibrils of Treponema refringens were studied in situ by electron microscopy of thin sectioned and negatively stained cells. From 5 to 21 parallel fibrils ran through the cell in a band adjacent to the inner side of the cytoplasmic membrane, on the inner sides of the curves of the spirochete. The nuclear areas of cells were adjacent to the fibrils. Cross sections of fibrils isolated from cells which had been lysed were polygonal and not uniformly electron dense. Polyacrylamide gel electrophoresis of partially purified fibril preparations indicated their main component to be a protein with a molecular weight of 97,000. Fibrils were solubilized by 1% trypsin, 1% pronase, 6 M urea, 1 N HCl, 0.005 N NaOH or 1.3% sodium dodecyl sulfate. By electron microscopy of negatively stained isolated fibrils, each fibril was found to be a complex arrangement of strands rather than a single tubule.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00423067
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