ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Proteins in the cytosol, post-nuclear particulate and nuclear fractions from dorsolateral and ventral prostates of rats were analyzed and compared by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Protein species separated in the gel were stained with Coomassie Brilliant Blue R-250 (CBB), and expressed as (apparent molecular weight of the respective species x 10-3)K. (i) In the cytosol fraction the contents of 30K, 67K, 110K, and 120K proteins were abundant in the dorsolateral prostate. On the other hand, 14K and 16K proteins were specific to the ventral prostate. By carbohydrate staining, major glycoproteins were found to be 120K and 16K for the dorsolateral and ventral prostates, respectively. (ii) In post-nuclear particulate fractions in both lobes, proteins having molecular weights greater than 42 000 were abundant. Of these, the contens of 65K, 105K and 120K were higher in the dorsolateral prostate than in the ventral prostate. Glycoproteins having the same apparent molecular weight as detected in the cytosol fractions were also found in the post-nuclear particulate fractions of both lobes. (iii) In the nuclear fraction H2A, H2B, H3 and H4 histones were fundamentally similar between the two lobes, whereas H1 histones including two subspecies were slightly but significantly higher in the ventral prostate than the dorsolateral prostate. The most remarkable difference between the lobes was the content of 20K in the dorsolateral prostate which was at least 20 times higher than that found in the ventral prostate.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150030509
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