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  • 1
    Electronic Resource
    Electronic Resource
    Flanking sequences of an Alu source stimulate transcription in vitro by interacting with sequence-specific transcription factors (1996)
    Springer
    Journal of molecular evolution 42 (1996), S. 30-36 
    Details
    ISSN: 1432-1432
    Keywords: Alu ; Transcription factors ; Flanking Sequence ; Retrotransposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An Alu source gene, called the EPL Alu, was previously isolated by a phylogenetic strategy. Sequences flanking the EPL Alu family member stimulate its RNA polymerase III (Pol III) template activity in vitro. One cis-acting element maps within a 40-nucleotide region immediately upstream to the EPL Alu. This same region contains an Apt site which, when mutated, abolishes the transcriptional stimulation provided by this region. The flanking sequence, as assayed by gel mobility shift, forms sequence-specific complexes with several nuclear factors including Apt. These results demonstrate that an ancestral Alu source sequence fortuitously acquired positive transcriptional control elements by insertion into the EPL locus, thereby providing biochemical evidence for a model which explains the selective amplification of Alu subfamilies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00163208
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  • 2
    Electronic Resource
    Electronic Resource
    DNA hybridization as a guide to phylogeny: Chemical and physical limits (1990)
    Springer
    Journal of molecular evolution 30 (1990), S. 237-246 
    Details
    ISSN: 1432-1432
    Keywords: DNA hybridization ; Duplex ; Thermal stability ; Sequence duplicates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of forming interspecific DNA heteroduplexes and estimating phylogenetic distances from the depression in their duplex melting temperature has several physical and chemical constraints. These constraints determine the maximum phylogenetic distance that may be estimated by this technique and the most appropriate method of analyzing that distance. Melting curves of self-renatured single copy primate DNAs reveal the presence of components absent from the renaturation products of exactly paired sequences. This observation, which confirms existing literature, challenges a fundamental assumption: that orthologous (i.e., corresponding) DNA sequences in the divergent species are being compared in DNA heteroduplex melting experiments. As a model system, the thermal stabilities of heteroduplexes formed between a human alpha-globin cDNA and four alpha-like globin genes isolated from chimpanzee are qualitatively compared. The results of this comparison show that the cross-hybrids of imperfectly matched gene duplicates from divergent species can contribute to the additional components that are present in renatured single copy DNAs. Single copy DNA, as usually defined, includes sequence duplicates that will obscure phylogenetic comparisons in a mass hybridization of genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099993
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