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  • AWI_EcolChem; Biological sample; BIOS; Ecological Chemistry @ AWI; Greenland; W_Greenland  (1)
  • Amphidoma languida; ARK-XXIX/1, TRANSSIZ; Azadinium; Azadinium poporum; Azadinium spinosum; AZAHAB; Azaspiracids; Baltic Sea; Cells, total; Celtic Sea; CT; CTD, towed system; CTD/Rosette; CTD-RO; CTD-twoyo; DATE/TIME; Dinoflagellates; DNA; English Channel; Event label; Field observation; HE516; HE516_10-2; HE516_1-1; HE516_11-1; HE516_12-1; HE516_13-1; HE516_14-1; HE516_15-1; HE516_16-1; HE516_17-1; HE516_18-1; HE516_19-1; HE516_20-1; HE516_21-1; HE516_2-2; HE516_22-1; HE516_23-1; HE516_24-1; HE516_25-1; HE516_26-1; HE516_27-1; HE516_28-1; HE516_29-1; HE516_30-1; HE516_3-1; HE516_31-1; HE516_32-1; HE516_33-1; HE516_34-1; HE516_35-1; HE516_36-1; HE516_37-1; HE516_38-1; HE516_39-1; HE516_40-1; HE516_4-1; HE516_41-1; HE516_42-1; HE516_43-1; HE516_44-1; HE516_45-1; HE516_46-1; HE516_47-1; HE516_48-1; HE516_49-1; HE516_50-2; HE516_5-1; HE516_51-1; HE516_52-1; HE516_53-1; HE516_54-1; HE516_55-1; HE516_56-1; HE516_57-1; HE516_58-1; HE516_59-1; HE516_60-1; HE516_6-1; HE516_61-1; HE516_62-1; HE516_63-1; HE516_64-1; HE516_65-1; HE516_66-1; HE516_67-1; HE516_68-1; HE516_69-2; HE516_70-1; HE516_7-1; HE516_71-1; HE516_72-1; HE516_73-1; HE516_74-2; HE516_75-2; HE516_8-1; HE516_9-2; HE517; HE517_10-1; HE517_11-3; HE517_1-2; HE517_12-1; HE517_13-2; HE517_14-1; HE517_15-1; HE517_16-1; HE517_17-1; HE517_19-1; HE517_21-1; HE517_22-1; HE517_23-1; HE517_25-1; HE517_26-1; HE517_27-1; HE517_28-1; HE517_30-1; HE517_35-2; HE517_36-2; HE517_37-1; HE517_8-1; HE517_9-1; HE534; HE534_11-3; HE534_1-4; HE534_22-3; HE534_28-4; HE534_30-2; HE534_33-4; HE534_36-4; HE534_38-5; HE534_42-5; HE534_4-3; HE534_43-1; HE534_47-2; HE534_8-4; HE541; HE541_105-1; HE541_36-1; HE541_57-1; HE541_75-1; Heincke; Kattegat; LATITUDE; LONGITUDE; North Atlantic; North Sea; Polarstern; PS92; PS92-track; qPCR; QPCR; Quantitative real-time PCR (qPCR); Reference/source; South Atlantic Ocean; The Great Belt; Underway cruise track measurements; UT1606; UT1606/01-1; UT1606/02-1; UT1606/03-1; UT1606/04-1; UT1606/05-1; UT1606/06-1; UT1606/07-1; UT1606/08-1; UT1606/09-1; UT1606/10-1; UT1606/11-1; UT1606/12-1; UT1606/13-1; UT1606/14-1; UT1606/15-1; UT1606/16-1; UT1606/17-1; UT1606/18-1; UT1606/19-1; UT1606/20-1; UT1606/21-1; UT1606/22-1; UT1606/23-1; UT1606/24-1; UT1606/25-1; UT1606/26-1; UT1606/27-1; UT1606/28-1; UT1606/29-1; UT1606/30-1; UT1606/31-1; UT1606/32-1; UT1606/33-1; UT1606/34-1; UT1606/35-1; UT1606/36-1; UT1606/37-1; UT1606/38-1; UT1606/39-1; UT1606/40-1; UT1606/41-1; UT1606/42-1; UT1606/43-1; UT1606/44-1; Uthörn  (1)
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  • 1
    Publication Date: 2024-05-11
    Description: The data represent species counts (cells L-1) of the three AZA-producing dinoflagellate species Azadinium spinosum, Az. poporum and Amphidoma languida (all members of the taxonomic family Amphidomataceae) of water samples taken during in total six different field expeditions on several research vessels (RV Heincke, RV Uthörn, RV Polarstern) and on in total five stationary sampling stations (Scapa Flow/Scotland, Cuxhaven/Germany, Helgoland/Germany, Wilhelmshaven/Germany, Sylt/Germany) between 2015 and 2019. The water samples have been taken using Niskin bottles (on research vessels attached to a CTD). After DNA extraction, the species cell numbers have been calculated by quantitative PCR (qPCR) analysis using respective standard curves. These samples gained from different geographical areas in the eastern North Atlantic have been analyzed as part of the RIPAZA Project (funded by the German BMBF; in cooperation with the Third Institute of Oceanography, Xiamen/China) and the results are presented and discussed in the doctoral thesis of Stephan Wietkamp (Suppl.Tab.S6, Suppl.Tab.S7). Aim of the project and especially of this data set was to provide first reference data on the biogeography (geographical distribution and seasonality) of toxigenic Amphidomataceae in the eastern North Atlantic.
    Keywords: Amphidoma languida; ARK-XXIX/1, TRANSSIZ; Azadinium; Azadinium poporum; Azadinium spinosum; AZAHAB; Azaspiracids; Baltic Sea; Cells, total; Celtic Sea; CT; CTD, towed system; CTD/Rosette; CTD-RO; CTD-twoyo; DATE/TIME; Dinoflagellates; DNA; English Channel; Event label; Field observation; HE516; HE516_10-2; HE516_1-1; HE516_11-1; HE516_12-1; HE516_13-1; HE516_14-1; HE516_15-1; HE516_16-1; HE516_17-1; HE516_18-1; HE516_19-1; HE516_20-1; HE516_21-1; HE516_2-2; HE516_22-1; HE516_23-1; HE516_24-1; HE516_25-1; HE516_26-1; HE516_27-1; HE516_28-1; HE516_29-1; HE516_30-1; HE516_3-1; HE516_31-1; HE516_32-1; HE516_33-1; HE516_34-1; HE516_35-1; HE516_36-1; HE516_37-1; HE516_38-1; HE516_39-1; HE516_40-1; HE516_4-1; HE516_41-1; HE516_42-1; HE516_43-1; HE516_44-1; HE516_45-1; HE516_46-1; HE516_47-1; HE516_48-1; HE516_49-1; HE516_50-2; HE516_5-1; HE516_51-1; HE516_52-1; HE516_53-1; HE516_54-1; HE516_55-1; HE516_56-1; HE516_57-1; HE516_58-1; HE516_59-1; HE516_60-1; HE516_6-1; HE516_61-1; HE516_62-1; HE516_63-1; HE516_64-1; HE516_65-1; HE516_66-1; HE516_67-1; HE516_68-1; HE516_69-2; HE516_70-1; HE516_7-1; HE516_71-1; HE516_72-1; HE516_73-1; HE516_74-2; HE516_75-2; HE516_8-1; HE516_9-2; HE517; HE517_10-1; HE517_11-3; HE517_1-2; HE517_12-1; HE517_13-2; HE517_14-1; HE517_15-1; HE517_16-1; HE517_17-1; HE517_19-1; HE517_21-1; HE517_22-1; HE517_23-1; HE517_25-1; HE517_26-1; HE517_27-1; HE517_28-1; HE517_30-1; HE517_35-2; HE517_36-2; HE517_37-1; HE517_8-1; HE517_9-1; HE534; HE534_11-3; HE534_1-4; HE534_22-3; HE534_28-4; HE534_30-2; HE534_33-4; HE534_36-4; HE534_38-5; HE534_42-5; HE534_4-3; HE534_43-1; HE534_47-2; HE534_8-4; HE541; HE541_105-1; HE541_36-1; HE541_57-1; HE541_75-1; Heincke; Kattegat; LATITUDE; LONGITUDE; North Atlantic; North Sea; Polarstern; PS92; PS92-track; qPCR; QPCR; Quantitative real-time PCR (qPCR); Reference/source; South Atlantic Ocean; The Great Belt; Underway cruise track measurements; UT1606; UT1606/01-1; UT1606/02-1; UT1606/03-1; UT1606/04-1; UT1606/05-1; UT1606/06-1; UT1606/07-1; UT1606/08-1; UT1606/09-1; UT1606/10-1; UT1606/11-1; UT1606/12-1; UT1606/13-1; UT1606/14-1; UT1606/15-1; UT1606/16-1; UT1606/17-1; UT1606/18-1; UT1606/19-1; UT1606/20-1; UT1606/21-1; UT1606/22-1; UT1606/23-1; UT1606/24-1; UT1606/25-1; UT1606/26-1; UT1606/27-1; UT1606/28-1; UT1606/29-1; UT1606/30-1; UT1606/31-1; UT1606/32-1; UT1606/33-1; UT1606/34-1; UT1606/35-1; UT1606/36-1; UT1606/37-1; UT1606/38-1; UT1606/39-1; UT1606/40-1; UT1606/41-1; UT1606/42-1; UT1606/43-1; UT1606/44-1; Uthörn
    Type: Dataset
    Format: text/tab-separated-values, 995 data points
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  • 2
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    Unknown
    PANGAEA
    In:  Supplement to: Sala-Pérez, Manuel; Alpermann, Tilman J; Krock, Bernd; Tillmann, Urban (2016): Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae). Harmful Algae, 55, 85-96, https://doi.org/10.1016/j.hal.2016.02.004
    Publication Date: 2024-04-14
    Description: Harmful algal blooms are mainly caused by marine dinoflagellates and are known to produce potent toxins that may affect the ecosystem, human activities and health. Such events have increased in frequency and intensity worldwide in the past decades. Numerous processes involved in Global Change are amplified in the Arctic, but little is known about species specific responses of arctic dinoflagellates. The aim of this work was to perform an exhaustive morphological, phylogenetical and toxinological characterization of Greenland Protoceratium reticulatum and, in addition, to test the effect of temperature on growth and production of bioactive secondary metabolites. Seven clonal isolates, the first isolates of P. reticulatum available from arctic waters, were phylogenetically characterized by analysis of the LSU rDNA. Six isolates were further characterized morphologically and were shown to produce both yessotoxins (YTX) and lytic compounds, representing the first report of allelochemical activity in P. reticulatum. As shown for one of the isolates, growth was strongly affected by temperature with a maximum growth rate at 15 °C, a significant but slow growth at 1 °C, and cell death at 25 °C, suggesting an adaptation of P. reticulatum to temperate waters. Temperature had no major effect on total YTX cell quota or lytic activity but both were affected by the growth phase with a significant increase at stationary phase. A comparison of six isolates at a fixed temperature of 10 °C showed high intraspecific variability for all three physiological parameters tested. Growth rate varied from 0.06 to 0.19 per day, and total YTX concentration ranged from 0.3 to 15.0 pg YTX/cell and from 0.5 to 31.0 pg YTX/cell at exponential and stationary phase, respectively. All six isolates performed lytic activity; however, for two isolates lytic activity was only detectable at higher cell densities in stationary phase.
    Keywords: AWI_EcolChem; Biological sample; BIOS; Ecological Chemistry @ AWI; Greenland; W_Greenland
    Type: Dataset
    Format: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet, 132.7 kBytes
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