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Methionine induction of ACV synthetase inCephalosporium acremonium (1987)

Zhang, Jin-you ; Banko, Gerald ; Wolfe, Saul ; [et al.]

Springer

Journal of industrial microbiology and biotechnology 2 (1987), S. 251-255

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ISSN: 1476-5535

Keywords: Methionine effect ; ACV accumulation ; β-Lactam production

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary Methionine markedly stimulates the biosynthesis of penicillin N and cephalosporin C inCephalosporium acremonium. Examination of intra- and extracellular ACV tripeptide in non-producing mutant N-2 showed that growth in the presence of methionine increased ACV accumulation. Direct measurement of ACV synthetase activity in a cell-free system indicated that the methionine effect was mainly due to induction of this first enzyme of the β-lactam biosynthetic pathway, resulting in a corresponding increase in β-lactam production in both a low-producing strain and a high-producing mutant.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01569547

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