ISSN:
1432-072X
Keywords:
2-Hydroxy
;
5-nitropenta
;
2,4-dienoic acid
;
Nitrite elimination
;
4-Nitropyrogallol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Both Alcaligenes eutrophus JMP 134 and its plasmid-free derivative Alcaligenes eutrophus JMP 222 utilize 2,6-dinitrophenol as sole source of carbon, energy, and nitrogen. In the presence of ammonia resting cells of these strains release two mol of nitrite per mol of 2,6-dinitrophenol. Alcaligenes eutrophus JMP 222-α1D, a mutant of strain JMP 222 obtained by transposon (Tn5) mutagenesis, is able to use 2,6-dinitrophenol as nitrogen source but not as source of carbon and energy. Resting cells of this mutant liberate only one mol of nitrite per mol of 2,6-dinitrophenol. A single metabolite was detected by high-pressure liquid chromatography and identified as 2-hydroxy-5-nitropenta-2,4-dienoic acid from the mass spectrum, the 1H-, and 13C-NMR spectra. Strain JMP 222-α1S, a spontaneous mutant of strain JMP 222-α1D, accumulates 4-nitropyrogallol which was identified as the initial metabolite of 2,6-dinitrophenol degradation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00245219
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