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  • 1
    Electronic Resource
    Electronic Resource
    Heat shock and light activation of aChlamydomonas HSP70 gene are mediated by independent regulatory pathways (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 727-734 
    Details
    ISSN: 1617-4623
    Keywords: Chlamydomonas reinhardtii ; HSP70 ; Light induction ; Inhibitors ; Promoter deletions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction ofHSP70 heat shock genes by light has been demonstrated inChlamydomonas. Our aim was to establish whether this induction by light is mediated by the heat stress sensing pathway or by an independent signal chain. Inhibitors of cytoplasmic protein synthesis revealed an initial difference. Cycloheximide and other inhibitors of protein synthesis preventedHSP70A induction upon illumination but not during heat stress. Analysis ofHSP70A induction in cells that had differentiated into gametes revealed a second difference. While heat shock resulted in elevatedHSP70A mRNA levels, light was no longer able to serve as an inducer in gametes. To identify the regulatory sequences that mediate the response of theHSP70A gene to either heat stress or light we introduced a series of progressive 5′ truncations into its promoter sequence. Analyses of the levels of mRNA transcribed from these deletion constructs showed that in most of them the responses to heat shock and light were similar, suggesting that light induction is mediated by a light-activated heat shock factor. However, we show that theHSP70A promoter also containscis-acting sequences involved in light induction that do not participate in induction by heat stress. Together, these results provide evidence for a regulation ofHSP70A gene expression by light through a heat shock-independent signal pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191713
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  • 2
    Electronic Resource
    Electronic Resource
    Locating the Position of Lithium in Solution by Combined 13C,6Li and 1H,6Li HOESY Measurements (1995)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 128 (1995), S. 799-802 
    Details
    ISSN: 0009-2940
    Keywords: 13C,6Li HOESY ; 1H,6Li HOESY ; Organolithium compounds ; Distance determination ; Spin lattice relaxation ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 13C,6Li HOESY Measurements were developed and performed for the first time on the 13C-labeled benzyllithium compound 1. 1D NOE difference, 1H,6Li HOESY, and spin lattice relaxation measurements were used to obtain information on the position of the lithium, ion in solution.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19951280810
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