Publication Date:
2011-03-10
Description:
Most plant-microbe interactions do not result in disease; natural products restrict non-host pathogens. We found that sulforaphane (4-methylsulfinylbutyl isothiocyanate), a natural product derived from aliphatic glucosinolates, inhibits growth in Arabidopsis of non-host Pseudomonas bacteria in planta. Multiple sax genes (saxCAB/F/D/G) were identified in Pseudomonas species virulent on Arabidopsis. These sax genes are required to overwhelm isothiocyanate-based defenses and facilitate a disease outcome, especially in the young leaves critical for plant survival. Introduction of saxCAB genes into non-host strains enabled them to overcome these Arabidopsis defenses. Our study shows that aliphatic isothiocyanates, previously shown to limit damage by herbivores, are also crucial, robust, and developmentally regulated defenses that underpin non-host resistance in the Arabidopsis-Pseudomonas pathosystem.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fan, Jun -- Crooks, Casey -- Creissen, Gary -- Hill, Lionel -- Fairhurst, Shirley -- Doerner, Peter -- Lamb, Chris -- Biotechnology and Biological Sciences Research Council/United Kingdom -- New York, N.Y. -- Science. 2011 Mar 4;331(6021):1185-8. doi: 10.1126/science.1199707.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉John Innes Centre, Norwich NR4 7UH, UK. jun.fan@bbsrc.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21385714" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/genetics/*metabolism/*microbiology
;
Bacterial Proteins/genetics/metabolism
;
Escherichia coli/drug effects/genetics/growth & development
;
*Genes, Bacterial
;
Glucosinolates/metabolism
;
*Host-Pathogen Interactions
;
Isothiocyanates/metabolism/pharmacology
;
Operon
;
Plant Diseases/microbiology
;
Plant Extracts/pharmacology
;
Plants, Genetically Modified
;
Pseudomonas syringae/drug effects/*genetics/growth & development/pathogenicity
;
Thiocyanates/isolation & purification/*metabolism/*pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
