Publication Date:
1998-08-26
Description:
As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other loci with high resolution (11 to 64 kilobases).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Winzeler, E A -- Richards, D R -- Conway, A R -- Goldstein, A L -- Kalman, S -- McCullough, M J -- McCusker, J H -- Stevens, D A -- Wodicka, L -- Lockhart, D J -- Davis, R W -- 1R01 HG01633/HG/NHGRI NIH HHS/ -- HG00185-01/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 1998 Aug 21;281(5380):1194-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA. winzeler@cmgm.stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9712584" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Chromosome Mapping/*methods
;
Cycloheximide/pharmacology
;
Drug Resistance, Microbial/genetics
;
Drug Resistance, Multiple/genetics
;
Gene Deletion
;
Genes, Fungal
;
Genetic Linkage
;
Genetic Markers
;
*Genetic Techniques
;
*Genetic Variation
;
*Genome, Fungal
;
Genotype
;
Nucleic Acid Hybridization
;
Phenotype
;
Recombination, Genetic
;
Saccharomyces cerevisiae/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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