ISSN:
0021-9304
Keywords:
microencapsulation
;
immunoadsorption
;
anti-A antibodies
;
double emulsion-evaporation
;
erythrocyte-ghosts
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
Large volume plasma exchanges are used for the removal of anti-Aor anti-B antibodies from the plasma of patients undergoingtransplantation from donors with major ABO incompatibility. Previous works suggest that solid-phase immunoadsorption can besubstituted for plasma exchange in situations where antigens canbe purified and immobilized on columns through which plasma ispercolated. However, the preparation of purified antigens of theABO system in large quantities is laborious and requires the useof considerable blood volumes. Studies were therefore undertakento determine the feasibility of an original immunoadsorbent basedon porous microparticles prepared by a water/oil/wateremulsification-solvent evaporation method, within whicherythrocytes-ghosts carrying blood-group antigens were entrapped. The decrease of the antibody hemagglutinating titre afteradsorption onto encapsulated ghosts suggests that antibodies cancross the polymeric membrane and bind to the antigens. Thisoriginal approach of using encapsulated antigens for the batchwiseremoval of antibodies could be extended to affinity chromatography, and immunoadsorption therapy with a chromatographic column linked to an extracorporeal circulation could be considered. © 1997John Wiley & Sons, Inc. J Biomed Mater Res, 37,155-160, 1997.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
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