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  • 1
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    Ultrastructure and histochemistry of Citrus limon (L.) stigma (1982)
    Springer
    Details
    Publication Date: 1982-11-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 2
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    Germination and early tube development in vitro of Lycopersicum peruvianum pollen: Ultrastructural features (1977)
    Springer
    Details
    Publication Date: 1977-01-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 3
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    Ultrastructural investigations on Lycopersicum peruvianum pollen activation and pollen tube organization after self-and cross-pollination (1980)
    Springer
    Details
    Publication Date: 1980-11-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 4
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    Alcuni cenni sulla biologia del polline (1985)
    Springer
    Details
    Publication Date: 1985-12-01
    Print ISSN: 0393-5965
    Electronic ISSN: 1573-3025
    Topics: Biology
    Published by Springer
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  • 5
    Electronic Resource
    Electronic Resource
    Germination and early tube development in vitro of Lycopersicum peruvianum pollen: Ultrastructural features (1977)
    Springer
    Planta 136 (1977), S. 239-247 
    Details
    ISSN: 1432-2048
    Keywords: Early tube development ; In vitro germination ; Lycopersicum ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphologic changes occurring during pollen grain activation and ultrastructural features of Lycopersicum peruvianum Mill. pollen tube during the first stages of growth in vitro have been studied. The more evident morphologic changes during activation, in comparison to those already described for mature inactive pollen, concern dictyosomes, rough endoplasmic reticulum (RER), and ribosomes. The dictyosomes are very abundant and produce “large” and “small” vesicles. Near the germinative pores both types of vesicles are present, while all along the remaining cell wall only the large type is observed. These latter react weakly to Thiéry's test and probably contain a callose precursor necessary for the deposition of a callosic layer lining at first only the inner side of the functioning pore and occasionally the other two pores, and subsequently the entire inner surface of the cell wall. The small vesicles, highly positive to Thiéry's test, are present only near the pores and could be involved in the formation of the pectocellulosic layer of the tube wall. The setting free of RER cisterns, which in the mature inactive pollen were aggregated in stacks, coinciding with polysome formation and resumption of protein synthesis, is in accord with the hypothesized role of RER cistern stacks as a reserve of synthesizing machinery. The pollen tube reaches a definitive spatial arrangement soon after the generative cell and vegetative nucleus have moved into it. At this stage four different zones that reflect a functional specialization are present. In the apical and subapical zone two types of dictysosome-originated vesicles, similar to those found in the activated pollen grain, are present. Their role in the formation of the callosic and pectocellulosic wall layers seems to be the same as in the activated pollen grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385991
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural features of Aloe ciliaris pollen (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 88-96 
    Details
    ISSN: 1432-2145
    Keywords: Pollen activation ; Generative cell ; Vegetative cell ; Cytoskeleton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Both the internal anatomy and the external morphology of the mature pollen grain of Aloe ciliaris have been studied, together with the cytological changes occurring during pollen activation. In mature pollen, the generative cell (GC) and the vegetative nucleus (VN) are closely associated with each other, and both can be found in the central part of the grain. In the generative cytoplasm, some organelles and microtubular bundles are present. In the vegetative cell, dictyosomes, stacks of rough endoplasmic reticulum, mitochondria, plastids, vacuoles, ribosomes, and masses of fibrillar material have been described. During pollen activation, important changes occur in both the generative and vegetative cells (VC). In the GC, the microtubular bundles become clearly visible, and the GC and VC gradually move towards the germ pore. The RER cisterns become free from the stacks, and organelles, such as dictyosomes, become very active. The fibrillar masses gradually decrease in number, and the individual fibrils become more evident and clearer in resolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189267
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  • 7
    Electronic Resource
    Electronic Resource
    The structure and cytochemistry of the pistil of Hypericum calycinum: the style (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 248-255 
    Details
    ISSN: 1432-2145
    Keywords: Hypericum calycinum ; Pistil ; Pollen pistil interaction ; Transmitting tissue ; Ultra structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A typical style of Hypericum calycinum is solid with a core of transmitting tissue traversing the whole length of the style. This transmitting tissue consists of loosely arranged cells and large intercellular spaces filled with a secretion product. The secretion product is rich in lipids, but poor in proteins and polysaccharides. The intercellular spaces of the transmitting tissue originate partly by a separation of cells as a result of the decomposition of the middle lamella and partly by degeneration of some of the cells of the transmitting tissue. H. calycinum is self-compatible. Both self- and cross-pollinations result in profuse pollen germination on the stigma and pollen tube growth through the style. The data on Hypericum is discussed in relation to information available on other solidstyled systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189160
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  • 8
    Electronic Resource
    Electronic Resource
    The ultrastructure of polymorphic pollen grains of Canna indica L. (1989)
    Springer
    Sexual plant reproduction 2 (1989), S. 193-198 
    Details
    ISSN: 1432-2145
    Keywords: Polymorphism ; Ultrastructure ; Pollen grains ; Canna indica L ; Tannin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Our investigations on Canna indica L. indicate that the pollen of this species is polymorphic: there are two types of pollen — a larger type and a comparatively smaller type. Transmission electron microscopy (TEM) revealed the presence of small vacuoles containing tannic substances in the generative cell (GC) of the larger grains: the GC of the mature grain contained a higher quantity of tannins than the GC of the immature grain. Mitochondria, lipid bodies, rough endoplasmic reticulum (RER) and microtubular bundles were present in the cytoplasm of the GC. Numerous mitochondria, lipid bodies and plastids were also present in the vegetative cell (VC), with the mitochondria clustered around the vegetative nucleus. The plastids were observed to be associated with the RER cisterns. During the maturation process, the number of starch grains contained in the plastids decreased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192766
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  • 9
    Electronic Resource
    Electronic Resource
    Plastid differentiation during Cucurbita pepo (Cucurbitaceae) pollen grain development (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 17-24 
    Details
    ISSN: 1432-2145
    Keywords: Cucurbita pepo ; Pollen ; Amyloplast ; Plastid division ; Plastid differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of plastids in the pollen of Cucurbita pepo was followed from meiosis to pollen maturation by quantitative light and electron microscopy. Plastids are initially undifferentiated, then divide, and at late microspore stage differentiate into amyloplasts containing starch. Later the amyloplasts form lobes and divide. Amyloplasts containing a single starch grain are present from the early bicellular stage. Plastid development is considered in relation to such cytoembryological features as the pollen does not dehydrate at anthesis and germination begins 3 min after pollination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230362
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural features of pollen tubes of Endymion non-scriptus modified by cytochalasin D (1991)
    Springer
    Sexual plant reproduction 4 (1991), S. 73-80 
    Details
    ISSN: 1432-2145
    Keywords: Pollen-tube structure ; Intracellular movement ; Tip-growth mechanism ; Cytochalasin effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytochalasins, known as inhibitors of various processes involving motility in plant and animal cells, induce far-reaching structural changes in the cytoplasm and walls of pollen tubes without destroying the capacity for subsequent growth in normal media. The fine structure of tubes of Endymion non-scriptus modified by cytochalasin D suggests that the changes all stem directly or indirectly from the interruption of the longrange cyclosis along the tube axis, which is sustained throughout the period of normal growth. The elimination of this movement breaks down the the pattern of flow responsible for the sorting-out process that maintains the characteristic zonation of organelles and other inclusions at the apex of the extending tube, and leads gradually to re-distribution of the vacuoles and membranes in the vegetative cell, the disposition of which is normally correlated with the longitudinally oriented flow pathways. Random local migrations of organelles and other inclusions of greater amplitude than is to be expected from Brownian movement continue in the tubes in the presence of cytochalasin D, indicating that the motility system is not wholly destroyed. Following the interruption of concerted axial movement, the polysaccharide wall-precursor bodies (P-particles), normally inserted into the wall mainly in the apical part of the tube during tip growth, gradually become dispersed throughout the tube and are incorporated in the wall at random, entering even into the intine of the parent pollen grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196491
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