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  • 1
    Electronic Resource
    Electronic Resource
    Exciton dynamics within growth islands of GaAs/Al x Ga1−x As single quantum wells (1995)
    Springer
    Il nuovo cimento della Società Italiana di Fisica 17 (1995), S. 1531-1536 
    Details
    ISSN: 0392-6737
    Keywords: Excitons and related phenomena (including electron-hole drops) ; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The localization dynamics of excitons within growth islands of GaAs/Al x Ga1−x As single quantum wells (SQW) have been investigated by time-resolved photoluminescence spectroscopy (TRPL). Several samples with different substrate misorientation and growth interruption times are compared with regard to the photoluminescence (PL) emission dynamics. For monolayer (ML) islands larger than the exciton radius,i.e. long growth interruption, the time evolution of the different PL peaks reflects the transfer of free excitons between growth islands as well as the localization within a single growth island. The samples with shorter growth interruption reveal a much less pronounced PL splitting due to ML islands. The dynamics appears to be mainly determined by exciton localization to thicker ML islands. The density of localization centres is larger for the sample grown on misoriented substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02457239
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  • 2
    Electronic Resource
    Electronic Resource
    Modified excitonic resonances in GaAs/AlAs multiple quantum well heterostructures with ultrathin barriers (1987)
    Springer
    Applied physics 44 (1987), S. 323-328 
    Details
    ISSN: 1432-0630
    Keywords: 78.65.-s ; 73.20.Dx ; 78.55.-m ; 73.60.Br
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Excitonic resonance structures in GaAs/AlAs multiple quantum well heterostructures with varying barrier-layer thicknessesL B down to 1.3 nm are investigated for two sets of samples with the nominal well widths ofL Z =9.2 and 6.4 nm, by 2K photoluminescence excitation spectroscopy. The observed resonance energies of then=1 heavyhole (1 hh) and light-hole (1 lh) free excitons imply that quantum confinement effects persist at least down to the decreased barrier-layer thickness ofL B =1.3 nm. This result is inconsistent with the red shifts expected from the simple well-coupling theory within the one-band Kronig-Penney model at theГ point. Instead, blue shifts of 6–8 meV (8–17 meV) are observed for the 1 hh (1 lh) excitonic resonance peaks whenL B is decreased from 10 to 2 nm. A relative decrease of the oscillator strength of the 1 lh transition compared to the 1 hh transition is also observed asL B is decreased. These results manifest important effects of the indirect-gap barrier material for the actual wavefunction matching across the interface and the breakdown of the envelope function approach to GaAs/AlAs quantum well heterostructures with ultrathin barriers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00624599
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  • 3
    Electronic Resource
    Electronic Resource
    High-energy dye laser pumped by wall-ablation lamps (1978)
    Springer
    Applied physics 15 (1978), S. 191-196 
    Details
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A guideline for the optimum design of linear wall-ablation lamps is presented with a simple empirical formula which describes the luminescent characteristics of lamps. The absolute luminescent efficiency of the wall-ablation lamp was measured. A high-energy dye laser with a quadruple-elliptical cylindrical cavity was constructed using these lamps, and an output energy of 23.3 J (11.6 MW) was obtained with an overall efficiency of 0.31 %. The energy-flow diagram of this laser system was computed with a rate-equation analysis, and the conversion efficiency from electrical input to laser output was determined. One can expect an overall efficiency of about 2 %.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00928207
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  • 4
    Electronic Resource
    Electronic Resource
    Intra-aortic prostaglandin E1 infusion in maturation of neuroblastoma (1982)
    Springer
    Cellular and molecular life sciences 38 (1982), S. 932-933 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01953660
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  • 5
    Electronic Resource
    Electronic Resource
    Toxicity of polychlorinated biphenyls increased with simultaneous ingestion of alkylbenzene sulfonic acid salt (1973)
    Springer
    Cellular and molecular life sciences 29 (1973), S. 822-823 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Nachweis, dass in PCB vergifteten Lebern von Ratten die Quantität des Cholesterins und die Aktivität des Anilin-Hydroxylase-Enzyms zunahm, während sich die Na−K−Mg-abhängige ATPase verminderte. Gleichzeitige Verabreichung von ABS bewirkte eine signifikante Steigerung der PCB-Vergiftung.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946309
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  • 6
    Electronic Resource
    Electronic Resource
    A new enzyme-linked immunosorbent assay (ELISA) for studying immunocytochemical procedures using an antiserum produced against spermidine as a model (1993)
    Springer
    Histochemistry and cell biology 99 (1993), S. 477-483 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Antiserum was produced in rabbits against the polyamine spermidine (Spd) conjugated to bovine serum albumin (BSA). The reactivity of the serum to Spd and a variety of structurally related compounds was quantified by a new immunocytochemical model system incorporating an enzyme-linked immunosorbent assay (ELISA) binding test. This is based on the principle of coupling these compounds to the wells of microtiter plate activated with poly-l-lysine and glutaraldehyde and incubating the wells by the indirect immunoperoxidase method. The antiserum showed a 25% cross reaction with spermine (Spm), putrescine (Put), and cadaverine (Cad), and a 1% cross reaction with 1,3-diaminopropane (Dap), but no cross reaction with monoacetyl polyamines and amino acids. The antibody binding was inhibited most effectively by absorption of the antiserum with N 1-acetylspermidine and Spd in the ELISA inhibition test. Also, immunoblot analysis of the antiserum with nitrocellulose paper gave completely identical results to the ELISA binding tests. Spd-like immunoreactivities in human melanoma BD and neuroblastoma IMR 32 cell lines are presented as examples of the staining pattern obtained with the antiserum. Absorption of the serum with N 1-acetylspermidine and Spd was demonstrated to abolish the immunostaining reaction. The immunohistochemical model is simple: amines and amino acids are bound in the same way as in aldehyde-fixed tissues and, in comparison to immunoblot analysis, the immunoreactivity can be more easily and accurately quantified by assay with the antibody. The model should prove useful in assessing the specificity of other antisera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274101
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  • 7
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of polyamines in the gastrointestinal tracts of rats and mice (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 465-471 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  An immunocytochemical method using a recently produced monoclonal antibody (ASPM-29) with an antibody specificity to spermine (Spm) and spermidine (Spd) fixed in situ, was used to demonstrate an immunocytochemical localization of polyamine (PA) pools in the gastrointestinal tracts of rats and mice. High PA immunoreactivity was always found in the cytoplasm of cells not only at the cell proliferative zone or the precursor cell zone but also at the neighboring non-proliferative premature cell zone of the epithelium, and a gradient of decreasing PA levels was noticed from these cells to the fully mature differentiated gastric surface mucous cells and absorptive cells of the small and large intestines. Also, strong staining for PAs was seen in the cytoplasm of fully differentiated gastric chief cells and neurons of both the myenteric and submucous plexuses, whereas the nuclei of the cells remained virtually unstained. These results may suggest that PAs are closely associated with the high biosynthetic activity in the cells of the gastrointestinal mucosa of normal rats and mice. This seems to be consistent with the PA imunocytochemical results previously obtained for neoplastic cells and active protein- or peptide-secreting cells, including exocrine or endocrine cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050065
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  • 8
    Electronic Resource
    Electronic Resource
    An evaluation of polyamine immunocytochemistry using immunocytochemical model systems (1994)
    Springer
    Histochemistry and cell biology 101 (1994), S. 287-294 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Polyamine (PA) immunocytochemistry (ICC) was evaluated by a recently developed enzyme-linked immunosorbent assay (ELISA) binding test (ELISABT) using an anti-spermine (Spm) serum raised against Spm conjugated via the cross-linker N-(γ-maleimidobutyryloxy)succinimide (GMBS) with bovine serum albumin. In the test the antiserum showed strong immunoreactivity with N 1-acetylspermine (Ac-Spm) and acetylspermidine (N 1-Ac-Spd and N 8-Ac-Spd), and low immunoreactivity with Spm and Spd, which was, however, markedly enhanced after reaction with GMBS, acetic anhydride or glutaraldehyde. Complete agreement with results of immunoblot analysis was observed. PA-like immunoreactivity observed in the present PA ICC in cells in the foveolae and isthmus of rat gastric glands was completely abolished by absorption of the serum with N 1,N 12-diacetyl-Spm, Ac-Spm or N 1-Ac-Spd, but not by Spm or Spd. This absorption test was then simulated by an ELISA inhibition test (ELISAIT) with a solid phase conjugated with Ac-Spm or Spm, and by a PA ICC model system using Sepharose gel beads conjugated with each of several PAs. The results strongly suggest that the immunostaining in the gastric mucosa was mainly due to antibody species in the serum specific to ‘acylated’ Spm and Spd, but not to Spm or Spd. Acetyl PAs exist at such low concentrations in animal tissues that they are virtually undetectable by current ICC methods. Therefore Spm and Spd are likely candidates for those detected, after having been converted by fixation into such PA derivatives as become reactive with the antiserum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315916
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  • 9
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies against glutaraldehyde-conjugated histamine: application to immunocytochemistry (1997)
    Springer
    Histochemistry and cell biology 107 (1997), S. 39-45 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We have developed mouse monoclonal antibodies (AHA-1–5, all IgG1 sub-isotype mAbs) against histamine (HA) conjugated to bovine serum albumin using glutaraldehyde-NaBH4. Among these, AHA-1 mAb was found to be the most useful for HA immunocytochemistry (ICC) in terms of specificity and sensitivity without non-specific immunobinding. AHA-1 was demonstrated to be specific to HA with an enzyme-linked immunosorbent assay (ELISA) binding test, simulating the ICC of tissue sections, and not reactive to any of the other amino acids and peptides with N-terminal histidine tested. By use of this antibody, indirect immunoperoxidase staining was observed in rat stomach fixed with glutaraldehyde (GA) in combination with NaBH4 reduction. In contrast, no immunoreactivity was seen in tissue fixed only with GA. Absorption controls indicated that the immunostaining could be completely inhibited by GA-conjugated HA, which was consistent with the results of an ELISA inhibition test. No cross-reactivity occurred with other GA-conjugated amino acids. ICC staining was dense in the cytoplasm of gastric enterochromaffin-like cells and very weak in mast cells. A new finding was that staining was noticed in some cell bands of the intermediate layer between the stratum lucidum and the stratum corneum of the stratified squamous epithelium of the gastric cardia, esophagus, tongue, and skin in rats. The results strongly suggest that the monoclonal antibody allowed highly specific detection of HA in animal tis- sues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050087
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  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of polyamines in the gastrointestinal tracts of rats and mice (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 465-471 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract An immunocytochemical method using a recently produced monoclonal antibody (ASPM-29) with an antibody specificity to spermine (Spm) and spermidine (Spd) fixed in situ, was used to demonstrate an immunocytochemical localization of polyamine (PA) pools in the gastrointestinal tracts of rats and mice. High PA immunoreactivity was always found in the cytoplasm of cells not only at the cell proliferative zone or the precursor cell zone but also at the neighboring non-proliferative premature cell zone of the epithelium, and a gradient of decreasing PA levels was noticed from these cells to the fully mature differentiated gastric surface mucous cells and absorptive cells of the small and large intestines. Also, strong staining for PAs was seen in the cytoplasm of fully differentiated gastric chief cells and neurons of both the myenteric and submucous plexuses, whereas the nuclei of the cells remained virtually unstained. These results may suggest that PAs are closely associated with the high biosynthetic activity in the cells of the gastrointestinal mucosa of normal rats and mice. This seems to be consistent with the PA imunocytochemical results previously obtained for neoplastic cells and active protein- or peptide-secreting cells, including exocrine or endocrine cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02473308
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