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  • TDC  (1)
  • cell suspension  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Greening and strictosidine lactam production in two cell lines of Vinca major cv. variegata (1987)
    Springer
    Plant cell, tissue and organ culture 8 (1987), S. 177-182 
    Details
    ISSN: 1573-5044
    Keywords: vinca major ; cell suspension ; culture ; chlorophyll content ; indole alkaloids ; strictosidine lactam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cell lines of Vinca major L. cv. variegata produced strictosidine lactam as the main alkaloid. Quantities varied from 500–1000 μg/g DW in the high-yielding line and 1–100 μg/g DW in the low-yielding line. Transfer of cells to alkaloid production medium resulted in a 6–8 fold increase in alkaloid production with the high-yielding line, some increase in the low-yielding line and in both lines induced intense greening (up to 200 μg chlorophyll/g DW) indicating chloroplast differentiation. Though in the source plant leaves are the main storage site, no correlation between alkaloid accumulation and chloroplast differentiation could be found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043154
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  • 2
    Electronic Resource
    Electronic Resource
    Elicitor-mediated induction of phenylalanine ammonia lyase and tryptophan decarboxylase: Accumulation of phenols and indole alkaloids in cell suspension cultures of Catharanthus roseus (1989)
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 71-78 
    Details
    ISSN: 1573-5044
    Keywords: Catharanthus ; elicitors ; PAL ; TDC ; phenols ; alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell suspension cultures (cell line No 615) of Catharanthus roseus cv. Little Delicata responded to elicitor treatment by accumulating monoterpenoid indole alkaloids and phenolic compounds. The excretion of phenols into the culture medium resulted from the induction of the branch-point enzyme phenylalanine ammonia lyase. The accumulation of alkaloids, however, occurred several hours earlier than the elicitor-mediated induction of tryptophan decarboxylase through which shikimate pathway intermediates are channelled into tryptamine and related indole alkaloids. The results indicate that both pathways for phenol and indole alkaloid biosynthesis responded to elicitor treatment and that no obvious causal relationship between pathways could be deduced from this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033466
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