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  • Salt tolerance  (5)
  • Genetic diversity  (3)
  • Springer  (7)
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  • Springer  (7)
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  • 1
    Electronic Resource
    Electronic Resource
    Salt tolerance in Lycopersicon species. I. Character definition and changes in gene expression (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 737-743 
    Details
    ISSN: 1432-2242
    Keywords: Salt tolerance ; Lycopersicon ; Yield ; Proteins ; C4 species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Salt tolerance defined in terms of fruit yield under different NaCl concentrations (171.1 and 325.1 mM) is analyzed in 11 lines belonging to: Lycopersicon esculentum, L. cheesmanii, L. chmielewski, L. peruvianum and L. pimpinellifolium. Four L. pimpinellifolium lines and two L. cheesmanii lines tolerated the 171.1mM treatment; the latter species even tolerates 325.1 mM of NaCl. Changes in gene expression induced by salt treatment were also investigated by studying anther and leaf zymograms for L. esculentum and one salt-tolerant L. pimpinellifolium line, and leaf proteinograms for all lines. Changes in leaf PRX and MDH enzymatic systems were detected, mainly in the salt-sensitive genotype (L. esculentum). Four saltrelated peptides from 14 500 to 40 000 daltons were found. A polyclonal antibody raised against one of these peptides (number 2), also binds another peptide, named 2′, of much higher molecular weight, present both in control and salt-tolerant L. cheesmanii lines at the end of 171.1 mM treatment. The xero-halophyte shrub Atriplex halimus also showed a likely 2′-homologous peptide with this treatment, while its counterpart C3 species A. triangularis did not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222664
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  • 2
    Electronic Resource
    Electronic Resource
    Salt tolerance in Lycopersicon species. III. Detection of quantitative trait loci by means of molecular markers (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 395-401 
    Details
    ISSN: 1432-2242
    Keywords: Salt tolerance ; Lycopersicon ; Yield ; QTLs ; Molecular markers ; MAS ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A segregating population derived from a cross between L. esculentum cv Madrigal and a line of L. pimpinellifolium was used to identify genetic markers linked to QTLs involved in salinity tolerance in terms of yield, under a conductivity of 15 dS/m (171.1 mM NaCl). Six markers resulted, associated with QTLs affecting average fruit weight, fruit number and total weight under salinity. One of them, Aco-1, behaves reversely to the expectation from parental means; this and other features make it a promising target to obtain salt-tolerant tomatoes. Epistatic interactions were also found, thus affecting the criteria for marker-assisted selection. Although only 41% of the loci assayed were polymorphic, a high efficiency in identifying QTLs was achieved, since 43% of the marker loci are linked to QTLs for the trait under study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223650
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  • 3
    Electronic Resource
    Electronic Resource
    Salt tolerance in Lycopersicon species. IV. Efficiency of marker-assisted selection for salt tolerance improvement (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 765-772 
    Details
    ISSN: 1432-2242
    Keywords: Salt tolerance ; Tomato breeding ; Marker-assisted selection ; Molecular markers ; QTL mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The usefulness of marker-assisted selection (MAS) to develop salt-tolerant breeding lines from a F2 derived from L. esculentum x L. pimpinellifolium has been studied. Interval mapping methodology of quantitative trait locus (QTL) analysis was used to locate more precisely previously detected salt tolerance QTLs. A new QTL for total fruit weight under salinity (TW) near TG24 was detected. Most of the detected QTLs [3 for TW, 5 for fruit number, (FN) and 4 for fruit weight (FW)] had low R 2 values, except the FW QTL in the TG180-TG48 interval, which explains 36.6% of the total variance. Dominant and overdominant effects were detected at the QTLs for TW, whereas gene effects at the QTLs for FJV and FW ranged from additive to partial dominance. Phenotypic selection of F2 familes and marker-assisted selection of F3 families were carried out. Yield under salinity decreased in the F2 generation. F3 means were similar to those of the F1 as a consequence of phentoypic selection. The most important selection response for every trait was obtained from the F3 to F4 where MAS was applied. While F3 variation was mainly due to the within-family component, in the F4 the FN and FW between-family component was larger than the within-family one, indicating an efficient compartmentalization and fixation of QTLs into the F4 families. Comparison of the yield of these families under control versus saline conditions showed that fruit weight is a key trait to success in tomato salt-tolerance improvement using wild Lycopersicon germplasm. The QTLs we have detected under salinity seem to be also working under control conditions, although the interaction family x treatment was significant for TW, thereby explaining the fact that the selected families responded differently to salinity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224074
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic diversity in the orange subfamily Aurantioideae. I. Intraspecies and intragenus genetic variability (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 599-609 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Citrus ; Fortunella ; Poncirus ; Isozymes ; Genetic diversity ; Germ plasm bank ; Heterozygosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Despite the great economic importance of citrus, its phylogeny and taxonomy remain a matter of controversy. Moreover pathogens of increased virulence and dramatic environmental changes are currently spreading or emerging. The objectives of the present paper, measuring genetic variability and studying its pattern of distribution, are crucial steps to optimize sampling strategies in the search of genotypes that tolerate or resist these threatening factors within the huge array of Citrus and Citrus related species. Their intraspecific and intrageneric variability was studied comparatively by means of ten enzymatic systems using eight different measures. The analysis of ten enzymatic systems allowed us to distinguish all the species and all but one artificial hybrid. The species with the lowest genotypic variability are C. myrtifolia, C. deliciosa (willow leaf mandarin), C. paradisi (grapefruit), C. limon (lemon) and C. sinensis (sweet orange), while Severinia buxifolia shows the highest value. A broad spectrum of heterozygosity values was found in the collection. Lemons (C. limon, C. meyeri, C. karna, C. volkameriana), limes (C. aurantifolia, C. limettioides, C. lattifolia) and C. bergamia show a very high percentage of heterozygosity which indicates an origin through interspecific hybridization. Two main factors limit genetic intraspecific variability: apomictic reproduction, where nucellar embryos are much more vigorous than the zygotic ones, and nurserymen selecting against variability in the seedling stage of the rootstocks or in propagating the scion cultivars vegetatively. Additionally, self-pollination appears in some species mainly used as rootstocks which would explain their low heterozygosity values. Genetic differences between species and genera are in general high, which suggests that adaptation might have played an important role during the evolution of the orange subfamily.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050169
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic variability in Lycopersicon species and their genetic relationships (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 113-120 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon ; Isozymes ; Genetic diversity ; Genetic distance ; Salt tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic diversity has to be described and measured in order to establish breeding strategies and manage genetic resources. It is also fundamental to develop a comparative intraspecific study before attempting to discuss and conclude any phylogenetic relationship. The genetic variability of Lycopersicon species was studied using starch gel electrophoresis of 11 enzymatic systems in a hierarchical fashion. The species with the greatest genetic variability are L. chilense, L. peruvianum and L. pennellii, mainly due to the within-line component. L. chmielewskii, L. parviflorum and L. pimpinellifolium show an intermediate total variability and their between-component clearly predominates over the within-component. The least variable species are L. cheesmanii and L. esculentum. Cluster analysis resulted in three main groups: one formed by the cultigen, L. pimpinellifolium, L. cheesmanii and L. peruvianum;another by two species with self-incompatibility systems, L. pennelli and L. chilense; and another by two autogamous species L. chmielewskii and L. parviflorum. With respect to L. esculentum the farthest related species is Solanum rickii and the closest, L. pimpinellifolium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223815
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  • 6
    Electronic Resource
    Electronic Resource
    Salt tolerance in Lycopersicon species. II. Genetic effects and a search for associated traits (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 769-774 
    Details
    ISSN: 1432-2242
    Keywords: Salt tolerance ; Lycopersicon ; Heritability ; Genetic correlations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eleven quantitative traits, mostly related to tomato plant growth and fruit set, and their association with salt tolerance in terms of fruit yield under a 171.1 mM NaCl treatment have been investigated in 206 progeny derived from an interspecific hybrid, L. esculentum x L. pimpinellifolium, by self-pollination. None of the traits were highly correlated phenotypically to salt tolerance; however, the immunologically-detected presence of peptide 2′ was significantly associated with high total fruit weight (TW) and number (FN) under saline treatment. Broad-sense heritability was estimated for these two salt-tolerance components as 53.44 and 72.59 %, respectively. Non-additive gene effects, which have to be considered in a breeding program for salt tolerance, have been detected in TW, FN and in average fruit weight (FW). Given that different types of gene action have been found depending on the presence or absence of a high NaCl concentration in the nutrient solution, a different set of genes, or genes, differently regulated, must be involved in the expression of TW, FN and other fruit-related characters depending on this environmental condition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222668
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic diversity in the orange subfamily Aurantioideae. I. Intraspecies and intragenus genetic variability (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 599-609 
    Details
    ISSN: 1432-2242
    Keywords: Citrus ; Fortunella ; Poncirus ; Isozymes ; Genetic diversity ; Germ plasm bank ; Heterozygosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Despite the great economic importance of citrus, its phylogeny and taxonomy remain a matter of controversy. Moreover pathogens of increased virulence and dramatic environmental changes are currently spreading or emerging. The objectives of the present paper, measuring genetic variability and studying its pattern of distribution, are crucial steps to optimize sampling strategies in the search of genotypes that tolerate or resist these threatening factors within the huge array of Citrus and Citrus related species. Their intraspecific and intrageneric variability was studied comparatively by means of ten enzymatic systems using eight different measures. The analysis of ten enzymatic systems allowed us to distinguish all the species and all but one artificial hybrid. The species with the lowest genotypic variability are C. myrtifolia, C. deliciosa (willow leaf mandarin), C. paradisi (grapefruit), C. limon (lemon) and C. sinensis (sweet orange), while Severinia buxifolia shows the highest value. A broad spectrum of heterozygosity values was found in the collection. Lemons (C. limon, C. meyeri, C. karna, C. volkameriana), limes (C. aurantifolia, C. limettioides, C. lattifolia) and C. bergamia show a very high percentage of heterozygosity which indicates an origin through interspecific hybridization. Two main factors limit genetic intraspecific variability: apomictic reproduction, where nucellar embryos are much more vigorous than the zygotic ones, and nurserymen selecting against variability in the seedling stage of the rootstocks or in propagating the scion cultivars vegetatively. Additionally, self-pollination appears in some species mainly used as rootstocks which would explain their low heterozygosity values. Genetic differences between species and genera are in general high, which suggests that adaptation might have played an important role during the evolution of the orange subfamily.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224564
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