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  • Antiviral agents  (2)
  • potato  (2)
  • Springer  (4)
  • 1
    Electronic Resource
    Electronic Resource
    A ribozyme gene and an antisense gene are equally effective in conferring resistance to tobacco mosaic virus on transgenic tobacco (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 329-338 
    Details
    ISSN: 1617-4623
    Keywords: Key words RNA cleavage ; Hammerhead ribozymes ; Antisense RNA ; Antiviral agents ; In vivo expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Ribozymes of the hammerhead class can be designed to cleave a target RNA in a sequence-specific manner and can potentially be used to specifically modulate gene activity. We have targeted the tobacco mosaic virus (TMV) genome with a ribozyme containing three catalytic hammerhead domains embedded within a 1 kb antisense RNA. The ribozyme was able to cleave TMV RNA at all three target sites in vitro at 25° C. Transgenic tobacco plants were generated which expressed the ribozyme or the corresponding antisense constructs directed at the TMV genome. Six of 38 independent transgenic plant lines expressing the ribozyme and 6 of 39 plant lines expressing the antisense gene showed some level of protection against TMV infection. Homozygous progeny of some lines were highly resistant to TMV; at least 50% of the plants remained asymptomatic even when challenged with high levels of TMV. These plants also displayed resistance to infection with TMV RNA or the related tomato mosaic virus (ToMV). In contrast, hemizygous plants of the same lines displayed only very weak resistance when inoculated with low amounts of TMV and no resistance against high inoculation levels. Resistance in homozygous plants was not overcome by a TMV strain which was altered at the three target sites to abolish ribozyme-mediated cleavage, suggesting that the ribozyme conferred resistance primarily by an antisense mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174391
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A ribozyme gene and an antisense gene are equally effective in conferring resistance to tobacco mosaic virus on transgenic tobacco (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 329-338 
    Details
    ISSN: 1617-4623
    Keywords: RNA cleavage ; Hammerhead ribozymes ; Antisense RNA ; Antiviral agents ; In vivo expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ribozymes of the hammerhead class can be designed to cleave a target RNA in a sequence-specific manner and can potentially be used to specifically modulate gene activity. We have targeted the tobacco mosaic virus (TMV) genome with a ribozyme containing three catalytic hammerhead domains embedded within a 1 kb antisense RNA. The ribozyme was able to cleave TMV RNA at all three target sites in vitro at 25°C. Transgenic tobacco plants were generated which expressed the ribozyme or the corresponding antisense constructs directed at the TMV genome. Six of 38 independent transgenic plant lines expressing the ribozyme and 6 of 39 plant lines expressing the antisense gene showed some level of protection against TMV infection. Homozygous progeny of some lines were highly resistant to TMV; at least 50% of the plants remained asymptomatic even when challenged with high levels of TMV. These plants also displayed resistance to infection with TMV RNA or the related tomato mosaic virus (ToMV). In contrast, hemizygous plants of the same lines displayed only very weak resistance when inoculated with low amounts of TMV and no resistance against high inoculation levels. Resistance in homozygous plants was not overcome by a TMV strain which was altered at the three target sites to abolish ribozyme-mediated cleavage, suggesting that the ribozyme conferred resistance primarily by an antisense mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174391
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A method to optimize N-application in relation to soil supply of N, and yield of potato (1993)
    Springer
    Plant and soil 154 (1993), S. 139-144 
    Details
    ISSN: 1573-5036
    Keywords: fertilizer ; optimum application ; plant nitrogen ; potato ; soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mathematical models of crop growth can provide estimates of the potential yield of potato, and also the minimum, critical N-concentration required, [Nc], to attain that yield. Efficient use of nitrogen requires that the crop incorporates sufficient nitrogen to attain its potential yield and that excess uptake is avoided. Predictions of the rate of supply of nitrogen from the soil are imprecise and so it has been difficult to estimate accurately the required application of fertilizer-N. Our work has shown the feasibility of using the growing crop as a monitor of the rate of supply of N from the soil. Using a low initial application rate of N at planting and monitoring uptake rate, we can estimate the contribution from the soil, and couple that information with estimates of yield and the related [Nc] to give an estimate of the requirement for supplementary applied-N. The method can be seen, therefore, as a means to determine the size of a second or subsequent part of a split application of fertilizer. This approach avoids much of the uncertainty over the fate of applied nitrogen and should offer growers the double benefits of economic use of fertilizer and of minimizing leaching losses. Further, by tailoring applications of N-fertilizer to the crop's requirements the grower will be better able to ensure the quality considerations in his crop.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011083
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  • 4
    Electronic Resource
    Electronic Resource
    A critical assessment of the value of petiole sap analysis in optimizing the nitrogen nutrition of the potato crop (1995)
    Springer
    Plant and soil 172 (1995), S. 247-260 
    Details
    ISSN: 1573-5036
    Keywords: petiole sap ; plant nitrogen ; potato ; sap tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The importance of standardizing on sampling procedures was shown by examining diurnal variation of petiole sap nitrate-N concentration, [NO3 --N], and its variation within the plant. There was important variability in petiole sap [NO3 --N] about the general decline in values through the season, in time, between cultivars, between nitrogen fertilizer treatments, and between replicates. We have shown the lack of a consistent relation between petiole sap [NO3 --N] and rates of uptake of nitrogen either in the period around the time of sampling, or integrated over the whole growing season. Finally, we have examined the literature and have been unable to find reports of critical, independent tests of the use of petiole sap [NO3 -] to guide the application of fertilizer supplements. We conclude that a new, critical attitude should be taken to testing petiole sap [NO3 -] if the technique is to be used properly and to its seeming potential. Comparisons were made between measurements using a hand-held system and an auto-analyser.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011327
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