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  • Agrobacterium rhizogenes  (1)
  • plant cell culture  (1)
  • Springer  (2)
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  • Springer  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Bioreactors for surface-immobilized cells (1995)
    Springer
    Plant cell, tissue and organ culture 42 (1995), S. 81-90 
    Details
    ISSN: 1573-5044
    Keywords: Adhesion ; bioreactor ; immobilization ; plant cell culture ; secondary metabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Surface immobilization of plant cells avoids the problem of hydrodynamic or shear stress, which tends to be characteristic of suspended cells cultured in typical, mechanically agitated bioreactor systems. Surface immobilization also promotes the natural tendency for plant cells to aggregate, which may improve the synthesis and accumulation of secondary metabolites. In addition, exchange of medium is made simple in surface-immobilized systems, and extracellular secondary products are easily recovered on a continuous basis. However, problems related to regulation of the thickness of the immobilized cell layer, maintenance of the biomass in a productive condition, and vacuolar retention of secondary products have yet to be resolved satisfactorily. This review focusses on two surface-immobilization technologies, differing primarily in the nature and the configuration of the inert support. Prototypes of these designs have been applied to a variety of plant cell systems at bioreactor volumes up to 20 litres. Results obtained with several alternative technologies are also summarized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037685
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  • 2
    Electronic Resource
    Electronic Resource
    Indole alkaloid production by hairy root cultures of Catharanthus roseus (1989)
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 79-93 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium rhizogenes ; Catharanthus roseus ; hairy root cultures ; indole alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy root cultures of Catharanthus roseus were established by infection with six different Agrobacterium rhizogenes strains. Two plant varieties were used and found to exhibit significantly different responses to infection. Forty-seven hairy root clones derived from normal plants and two derived from the flowerless variety were screened for their growth and indole alkaloid production. The growth rate and morphological appearance showed wide variations between the clones. The alkaloid spectra observed were qualitatively but not quantitatively very similar to that of the corresponding normal plant roots. No vindoline or deacetyltransferase activity could be detected in any of the cultures studied. O-acetylval-lesamine, an alkaloid which has not been previously observed in C. roseus was identified from extracts of hairy root clone No. 8. Two root clones were examined for their growth and alkaloid accumulation during a 26-day culture period. Alkaloid accumulation parallelled growth in both clones with ca. 2 mg ajmalicine and catharanthine per g dry weight being observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033467
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    Paper (German National Licenses)
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