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  • 1
    Electronic Resource
    Electronic Resource
    Arsenic and copper residues in waterbirds and their food down inlet from the Island Copper Mill (1992)
    Springer
    Bulletin of environmental contamination and toxicology 48 (1992), S. 733-738 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195995
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of 15N-isotope dilution for measurement of nitrogen fixation in chickpea (Cicer arietinum L.) (1988)
    Springer
    Biology and fertility of soils 6 (1988), S. 347-351 
    Details
    ISSN: 1432-0789
    Keywords: N2-fixation ; 15N ; Cicer arietinum ; Isotope-dilution ; Acetylene reduction assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary N accumulation, nodulation, and acetylene reduction activity were measured at frequent intervals during the growth of two chickpea genotypes, and N2 fixation was estimated by an isotope-dilution method, using safflower as a non-N2-fixing reference. Safflower was more efficient at N uptake than both the chickpea genotypes for at least the first 50 days and thus could not be used as an accurate reference control. We recommend that further work should employ non-nodulatiog genotypes of chickpea as reference plants and use slow-release forms of 15N fertilizer. Direct genotype comparison by isotope dilution estimated that genotype K 850 fixed 16–18 kg ha−1 more N than G 130, and this difference was supported by the greater nodule mass and acetylene reduction activity in the K 850 cultivar. Inoculation with an ineffective chickpea Rhizobium sp. led to 69% nodulation on cultivar K 850 but only 33% on G 130. While nodule weight, N uptake, and acetylene reduction activity decreased with inoculation in K 850, the isotope dilutions were similar for both inoculation treatments. The lack of a significant effect on N2 fixation was ascribed to the partial success of inoculant establishment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261025
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  • 3
    Electronic Resource
    Electronic Resource
    Metal-binding protein in the Pacific Oyster,Crassostrea gigas: Assessment of the protein as a biochemical environmental indicator (1987)
    Springer
    Bulletin of environmental contamination and toxicology 38 (1987), S. 707-714 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01608607
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  • 4
    Electronic Resource
    Electronic Resource
    Binding of Endogenous Copper and Zinc to Cadmium-Induced Metal-Binding Proteins in Various Tissues of Perna viridis (1996)
    Springer
    Archives of environmental contamination and toxicology 30 (1996), S. 267-273 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract. Green lipped mussels, Perna viridis, were exposed to cadmium chloride (CdCl2; 0.52 and 1 μg/ml) in water for 4 days. The concentrations of cadmium (Cd), copper (Cu) and zinc (Zn) were measured in the viscera, gill, gonads, mantle, and muscle. There was a significant increase (p〈0.05) in Cd concentration in all tissues studied. Results from Sephadex G-75 chromatography indicated that most Cd was bound to a fraction of heat-stable proteins similar to the metal-binding protein (MBP) metallothionein. After exposure to Cd, there was no significant change in Cu concentration in total tissue proteins or in total cytosolic proteins. A significant increase (p〈0.05) in Cu, however, was detected in heat-stable proteins bound to the Cd-induced MBP in both viscera and gill. Copper bound to MBP also occurred in the gonad, mantle, and muscle, but to a much lesser extent. These results showed that Cd-induced MBP can also bind endogenous Cu. Zinc concentration in total heat-stable protein was increased only in gill and the muscle. Unlike Cu, a small amount of Zn binding to Cd-induced MBP was detected only in these tissues. Considering that both Cu and Zn exist intracellularly in dynamic equilibrium, the binding of Cu, but not Zn, to MBP may be explained by the kinetic reactivity of the two different metals to protein. The results of this study support the thesis that induction of intracellular MBP may also bind endogenous Cu and Zn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215807
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  • 5
    Electronic Resource
    Electronic Resource
    Binding of endogenous copper and zinc to cadmium-induced metal-binding proteins in various tissues of Perna viridis (1996)
    Springer
    Archives of environmental contamination and toxicology 30 (1996), S. 267-273 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Green lipped mussels, Perna viridis, were exposed to cadmium chloride (CdCl2; 0.52 and 1 μg/ml) in water for 4 days. The concentrations of cadmium (Cd), copper (Cu) and zinc (Zn) were measured in the viscera, gill, gonads, mantle, and muscle. There was a significant increase (p〈0.05) in Cd concentration in all tissues studied. Results from Sephadex G-75 chromatography indicated that most Cd was bound to a fraction of heat-stable proteins similar to the metal-binding protein (MBP) metallothionein. After exposure to Cd, there was no significant change in Cu concentration in total tissue proteins or in total cytosolic proteins. A significant increase (p〈0.05) in Cu, however, was detected in heat-stable proteins bound to the Cd-induced MBP in both viscera and gill. Copper bound to MBP also occurred in the gonad, mantle, and muscle, but to a much lesser extent. These results showed that Cd-induced MBP can also bind endogenous Cu. Zinc concentration in total heat-stable protein was increased only in gill and the muscle. Unlike Cu, a small amount of Zn binding to Cd-induced MBP was detected only in these tissues. Considering that both Cu and Zn exist intracellularly in dynamic equilibrium, the binding of Cu, but not Zn, to MBP may be explained by the kinetic reactivity of the two different metals to protein. The results of this study support the thesis that induction of intracellular MBP may also bind endogenous Cu and Zn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215807
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  • 6
    Electronic Resource
    Electronic Resource
    Chromoplasts of Tropaeolum majus L.: Lipid synthesis in whole organelles and subfractions (1978)
    Springer
    Planta 141 (1978), S. 303-309 
    Details
    ISSN: 1432-2048
    Keywords: Acyl-CoA hydrolase ; Acyltransferase ; Chromoplasts ; Galactosyltransferase ; Tropaeolum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation of tubulous chromoplasts from Tropaeolum majus L. petals was achieved in pure form. Their main substructures-lipid bodies, tubules, and envelope membranes-have been enriched. Whole chromoplasts as well as substructures have been tested for their activities in lipid synthesis. The following activities were found: fatty acid synthesis from acetate, glycosyl transfer reactions from UDP-galactose and UDP-glucose to galactolipids and sterols, acyltransferase reactions from palmitoyl-CoA, and a very active acyl-CoA hydrolase (EC 3.1.2.2.). Fatty acid synthesis was restricted to whole chromoplasts. Glycosyl- and acyltransferases were essentially confined to envelope membranes, whereas acyl-CoA hydrolase was found in all fractions. The chemical composition of chromoplast subfractions was determined. The lipid bodies consisted mainly of galactolipids and carotenoid esters in a 1:1 ratio, together with small amounts of protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388348
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  • 7
    Electronic Resource
    Electronic Resource
    Electron microscopical localisation of the 23S and 16S rRNA genes within an inverted repeat for two chromoplast DNAs (1981)
    Springer
    Current genetics 4 (1981), S. 25-28 
    Details
    ISSN: 1432-0983
    Keywords: Chromoplasts ; DNA ; Inverted Repeats ; rRNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Circular plastid DNA molecules, isolated from flower chromoplasts of the daffodil (a monocotyledon), and the nasturtium (a dicotyledon), have been shown by electron microscopy to contain inverted repeat sequences of 28.5 ± 0.7 kbp and 27.1 ± 1.0 kbp, respectively. The regions separating the repeats have lengths of 16.6 ± 0.8 kb for the shorter region, and 87.8 ± 4.8 kb for the longer in the daffodil, and 18.5 ± 0.5 kb and 82.3 ± 3.1 kb for the corresponding regions in the nasturtium. Further, in both cases, the 23S and 16S ribosomal RNA genes have been located by hybridisation (R-Loop technique) within this inverted repeat. Although the distance between the 16S and 23S genes is comparable, the position of the rRNA gene blocks within the repeats is different in these two chromoplast DNAs, with a shift of 1,500 bp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376782
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  • 8
    Electronic Resource
    Electronic Resource
    The ribosomal RNA genes from chloroplasts of mustard (Sinapis alba L.): mapping and sequencing of the leader region (1984)
    Springer
    Plant molecular biology 3 (1984), S. 147-158 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast genome ; leader region ; rDNA ; Sinapis alba ; Zea mays ; tRNAVal gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes coding for rRNAs from mustard chloroplasts were mapped within the inverted repeat regions of intact ctDNA and on ctDNA fragments cloned in pBR322. R-loop analysis and restriction endonuclease mapping show that the genes for 16S rRNA map at distances of 17 kb from the junctions of the repeat regions with the large unique region. The genes for 23S rRNA are located at distances of 2.8 kb from the junctions with the small unique region. Genes for 4.5S and 5S rRNA are located in close proximity to the 23S rRNA genes towards the small unique region. DNA sequencing of portions of the 5′ terminal third from the mustard 16S rRNA gene shows 96–99% homology with the corresponding regions of the maize, tobacco and spinach chloroplast genes. Sequencing of the region proximal to the 16S rRNA gene reveals the presence of a tRNAVal gene in nearly the same position and with identical sequence as in maize, tobacco and spinach. Somewhat less but still strong homology is also observed for the tDNA Val/16S rDNA intercistronic regions and for the regions upstream of the tRNAVal gene. However, due to many small and also a few larger deletions and insertions in the leader region, common reading frames coding for homologous peptides larger than 44 amino acids can not be detected; it is therefore unlikely that this region contains a protein coding gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016062
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  • 9
    Electronic Resource
    Electronic Resource
    Isolation of heavy metal binding proteins from marine vertebrates (1974)
    Springer
    Marine biology 28 (1974), S. 83-86 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cadmium binding proteins have been isolated from liver homogenates of marine vertebrates by ultracentri fugation and gel filtration. Liver samples of the Atlantic grey seal Halichoerus grypus and the Pacific fur seal Callorhinus ursinus contain measurable quantities of cadmium binding protein. The copper rock fish Sebastodes caurinus showed an increase in hepatic cadmium binding protein on administration of CdCl2, in agreement with the known inducible nature of the protein isolated from terrestrial animals. The apparent molecular weights of the isolated proteins were 9000 for the grey seal, 10,000 for the fur seal, and 11,000 for the copper rock fish, as determined by gel filtration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396298
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  • 10
    Electronic Resource
    Electronic Resource
    The fine structure of the planarianPolycelis tenuis Iijima (1974)
    Springer
    Protoplasma 79 (1974), S. 1-17 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The fine structure of the Minotian gland cell (spherical or granular club cell) and the phagocytic cell of the gastrodermis is described. The Minotian cells characteristically contain distinctive granules which are produced by the Golgi. Inclusion bodies containing membranous remains can also be found in the cytoplasm. The phagocytic cells bear groups of microvilli near adjacent junctions and have a much infolded basement membrane. Near the pharynx these cells contain the rod-shaped apical bodies found in the pharyngeal epithelium. Further into the intestine the cells more characteristically contain numerous phagocytic vacuoles and vacuolar dense bodies. After feeding, a consistent occlusion of the intestinal lumen has been observed. The columnar phagocytes have been shown to phagocytose cellular material and will also take up peroxidase and thorium oxide. The substances initially appear in the apical phagosomes. Acid phosphatase activity has been detected in the phagosomes after feeding. The enzyme is packaged in the Golgi and occurs in Golgi vacuoles and lysosomes of similar dimensions (morphologically vacuolar dense bodies). The fusion of lysosomes with phagosomes has been demonstrated histochemically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02055779
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