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  • Blackwell Science Ltd  (1)
  • MDPI Publishing  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 33 (1999), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The mechanism of protein secretion mediated by the β-domain of the Neisseria gonorrhoeae IgA protease, a paradigm of a family of secreted polypeptides of Gram-negative bacteria called autotransporters, has been examined using a single-chain antibody (scFv) as a reporter passenger domain to monitor the translocation process. Fusion of a scFv to the β-module of the IgA protease allowed us to investigate the passage of the chimeric protein through the periplasm, its insertion into the outer membrane and the movement of the N-terminal moiety towards the cell surface. As the binding activity of the scFv to its target antigen is entirely dependent on the formation of disulphide bonds, the relationship between secretion, folding and formation of S–S bridges could be analysed in detail. In contrast to the current notion that only an unfolded N-passenger domain can be translocated through the β-domain, our results show that the scFv is able to pass through the outer membrane, albeit at a threefold reduced level, in an active conformation with its disulphide bonds preformed in the periplasm through the action of the DsbA product. These data call for a re-evaluation of the prevailing model for secretion of the N-domain of autotransporters.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2018-08-10
    Description: IJERPH, Vol. 15, Pages 1703: Development of a Geospatial Data-Based Methodology for Stormwater Management in Urban Areas Using Freely-Available Software International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081703 Authors: Cristina Allende-Prieto Beatriz I. Méndez-Fernández Luis A. Sañudo-Fontaneda Susanne M. Charlesworth Intense urbanisation, combined with climate change impacts such as increased rainfall intensity, is overloading conventional drainage systems, increasing the number of combined sewer overflow events and making treatment plants outdated. There is a need for better urban planning, incorporating stormwater and flood management design in order to accurately design urban drainage networks. Geographic Information System (GIS) tools are capable of identifying and delineating the runoff flow direction, as well as accurately defining small-sized urban catchments using geospatial data. This study explores the synergies between GIS and stormwater management design tools for better land-use planning, providing a new methodology which has the potential to incorporate hydraulic and hydrological calculations into the design of urban areas. From data collection to final results, only freely available software and open platforms have been used: the U.S. EPA Storm Water Management Model (SWMM), QGis, PostgreSQL, PostGIS, SagaGIS, and GrassGIS. Each of these tools alone cannot provide all the necessary functionalities for large-scale projects, but once linked to GISWATER, a unique, fast, efficient, and accurate work methodology results. A case study of a newly urbanised area in the city of Gijón (northern Spain) has been utilised to apply this new methodology.
    Print ISSN: 1661-7827
    Electronic ISSN: 1660-4601
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by MDPI Publishing
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