ISSN:
1573-5060
Keywords:
Esterase
;
acid phosphatase
;
peroxidase
;
banding pattern
;
Principal Component Analysis
;
genetic distance
;
tissue specificity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Electrophoretic isozyme technique was applied on primary leaf, stem, and root tissues from seedlings of 34 U.S. major common bean (Phaseolus vulgaris L.) cultivars belonging to 19 commercial classes (Great Northern, Kidney, Navy, Pinto, Red Mexican, Tropical Black, California Small White, Idaho Flat Small White, Pink, and Cranberry). Among the isozyme systems studied, peroxidase (PER) and esterase (EST) were found to be suitable for cultivar identification within most commercial classes and for estimating the genetic relationships among the cultivars of the same class or among the classes. Acid phosphatase (PHOS), due to high proportions of monomorphic bands, could not be considered a good system for such purposes. Within each isozyme system, no pattern was found to be exclusive to any particular commercial class. Based on the number of polymorphic bands in common between each cultivar pair, a banding-similarity index was calculated. The indices were found to be highly significantly correlated with genetic distances obtained by Principal Component Analysis (PCA). In those comparisons where a pedigree relationship could be calculated, a non-significant correlation with similarity indices was obtained. Certain cultivar pair relationships, a minority of the whole, were incorrectly predicted by the isozyme technique. Caution is indicated when this technique is the only basis of assigning relationship. In a few cases, the similarity indices pointed either to close genetic relationships or the lack of such relationships, whereas the reverse is known from pedigree or PCA distance estimates. The reason for such discrepancies is discussed. Some isozymes were unique to a certain tissue, while others were present in more than one. Upon the compilation of bands from all the cultivars, for the leaf, stem, and root tissues respectively. 6, 4, and 0 EST, 9, 10, and 8 PHOS, and 7, 6, and 7 PER bands were obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00023706
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