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  • 1
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    Separation of D and L amino acids by liquid chromatography: use of chiral eluants (1979)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1979-06-15
    Description: An aqueous eluant containing a chiral copper-proline complex effects the separation of underivatized amino acid enantiomers on an ion-exchange column. The stereoselectivity is ascribed to differences in stability of the diastereomeric amino acid-copper compexes formed in solution. A simple change in the chirality of the eluant reverses the order of the enantiomer elution. For detection and quantification of picomole amounts of amino acids, the eluant is monitored for fluorescence after reaction with o-phthalaldehyde, a reagent insensitive to proline but highly sensitive for amino acids containing a primary amino group.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hare, P E -- Gil-Av, E -- New York, N.Y. -- Science. 1979 Jun 15;204(4398):1226-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/36662" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acids/*isolation & purification ; Chromatography, Liquid/methods ; Copper ; Hydrogen-Ion Concentration ; Proline ; Stereoisomerism ; Temperature
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Chiral recognition by nucleosides and nucleotides: resolution of helicenes by high-performance liquid chromatography (1981)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1981-09-18
    Description: Chiral recognition by nucleosides and nucleotides coated on silica gel was studied by high-performance liquid chromatography. Helicenes, which are chiral polyaromatic hydrocarbons, were used as probes. Stereoselectivity was detected when the nucleobase was a purine (adenosine 3', 5'-monophosphate, an guanosine), but was not detected with the pyrimidine derivative uridine. For a given nucleobase (adenine), all changes in the ribose moiety affected the resolution factors, which ranged between 1.03 and 1.074. These results might be relevant to the enantioselectivity of carcinogenic metabolites of polyaromatic hydrocarbons.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Y H -- Tishbee, A -- Gil-Av, E -- New York, N.Y. -- Science. 1981 Sep 18;213(4514):1379-81.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7268441" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry, Physical ; Chromatography, High Pressure Liquid ; *Nucleosides ; *Nucleotides ; Physicochemical Phenomena ; *Polycyclic Compounds ; Stereoisomerism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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