Publication Date:
1988-07-22
Description:
The central portion (region P) of the 742-nucleotide noncoding 5' end of poliovirus allows the RNA to initiate protein synthesis in the absence of the usual 5' 7-methylguanosine capping group. Poliovirus 5' noncoding region was fused to a reporter gene and transfected into cells. There was extensive augmentation of the expression of this gene by poliovirus-mediated inhibition of cap-dependent protein synthesis. That the construct initiated in a cap-independent manner was verified through in vitro experiments. Small lesions throughout region P blocked its initiation function, implying that a coherent functional unit, hundreds of nucleotides long, is responsible for cap-independent initiation by poliovirus RNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Trono, D -- Pelletier, J -- Sonenberg, N -- Baltimore, D -- AI 22346/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1988 Jul 22;241(4864):445-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Whitehead Institute for Biomedical Research, Cambridge, MA 02142.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2839901" target="_blank"〉PubMed〈/a〉
Keywords:
Acetyltransferases/genetics
;
Animals
;
Cell Line
;
Cell-Free System
;
Cercopithecus aethiops
;
Chloramphenicol O-Acetyltransferase
;
DNA Mutational Analysis
;
DNA, Recombinant
;
Poliovirus/*genetics
;
Protein Biosynthesis
;
RNA Caps
;
RNA, Messenger/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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