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  • American Physical Society  (548)
  • American Association for the Advancement of Science (AAAS)  (30)
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  • 1
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    Epidermal devices for noninvasive, precise, and continuous mapping of macrovascular and microvascular blood flow (2015)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2015-10-31
    Beschreibung: Continuous monitoring of variations in blood flow is vital in assessing the status of microvascular and macrovascular beds for a wide range of clinical and research scenarios. Although a variety of techniques exist, most require complete immobilization of the subject, thereby limiting their utility to hospital or clinical settings. Those that can be rendered in wearable formats suffer from limited accuracy, motion artifacts, and other shortcomings that follow from an inability to achieve intimate, noninvasive mechanical linkage of sensors with the surface of the skin. We introduce an ultrathin, soft, skin-conforming sensor technology that offers advanced capabilities in continuous and precise blood flow mapping. Systematic work establishes a set of experimental procedures and theoretical models for quantitative measurements and guidelines in design and operation. Experimental studies on human subjects, including validation with measurements performed using state-of-the-art clinical techniques, demonstrate sensitive and accurate assessment of both macrovascular and microvascular flow under a range of physiological conditions. Refined operational modes eliminate long-term drifts and reduce power consumption, thereby providing steps toward the use of this technology for continuous monitoring during daily activities.
    Digitale ISSN: 2375-2548
    Thema: Allgemeine Naturwissenschaft
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Nanoscale magnetic imaging of ferritins in a single cell (2019)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2019
    Beschreibung: 〈p〉The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. With the scanning, intracellular ferritins are imaged with a spatial resolution of ca. 10 nm, and ferritin-containing organelles are colocalized by correlative MI and electron microscopy. The approach paves the way for nanoscale MI of intracellular proteins.〈/p〉
    Digitale ISSN: 2375-2548
    Thema: Allgemeine Naturwissenschaft
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
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    Genomic and functional evidence reveals molecular insights into the origin of echolocation in whales (2018)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2018
    Beschreibung: 〈p〉Echolocation allows toothed whales to adapt to underwater habitats where vision is ineffective. Because echolocation requires the ability to detect exceptional high-frequency sounds, fossils related to the auditory system can help to pinpoint the origin of echolocation in whales. However, because of conflicting interpretations of archaeocete fossils, when and how whales evolved the high-frequency hearing correlated with echolocation remain unclear. We address these questions at the molecular level by systematically investigating the convergent evolution of 7206 orthologs across 16 mammals and find that convergent genes between the last common ancestor of all whales (LCAW) and echolocating bats are not significantly enriched in functional categories related to hearing, and that convergence in hearing-related proteins between them is not stronger than that between nonecholocating mammalian lineages and echolocating bats. However, these results contrast with those of parallel analyses between the LCA of toothed whales (LCATW) and echolocating bats. Furthermore, we reconstruct the ancestral genes for the hearing protein 〈i〉prestin〈/i〉 for the LCAW and LCATW; we show that the LCAW 〈i〉prestin〈/i〉 exhibits the same function as that of nonecholocating mammals, but the LCATW 〈i〉prestin〈/i〉 shows functional convergence with that of extant echolocating mammals. Mutagenesis shows that functional convergence of prestin is driven by convergent changes in the prestins S392A and L497M in the LCATW and echolocating bats. Our results provide genomic and functional evidence supporting the origin of high-frequency hearing in the LCAW, not the LCATW, and reveal molecular insights into the origin and evolutionary trajectories of echolocation in whales.〈/p〉
    Digitale ISSN: 2375-2548
    Thema: Allgemeine Naturwissenschaft
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
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    Exocytosis in spermatozoa in response to progesterone and zona pellucida (1994)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1994-12-02
    Beschreibung: Exocytosis in mammalian spermatozoa (the acrosome reaction) is a process essential for fertilization. Both progesterone and zona pellucida induce exocytosis in spermatozoa, which may encounter both during penetration of the oocyte's vestments. When mouse spermatozoa were exposed first to progesterone and then to zona pellucida, exocytosis was enhanced to a greater degree than that seen when the agonists were presented together or in the inverse order, which suggests that the steroid exerts a priming effect. Progesterone similarly primed the generation of intracellular messengers evoked by zona pellucida. The effects triggered by progesterone were mimicked by gamma-aminobutyric acid (GABA) and were blocked by bicuculline, which indicates that the steroid acts on a GABAA receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roldan, E R -- Murase, T -- Shi, Q X -- New York, N.Y. -- Science. 1994 Dec 2;266(5190):1578-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Development and Signalling, Babraham Institute, Biotechnology and Biological Sciences Research Council, Cambridge, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7985030" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acrosome/drug effects/*physiology ; Animals ; Bicuculline/pharmacology ; Calcium/metabolism ; Diglycerides/metabolism ; *Exocytosis/drug effects ; Male ; Mice ; Phosphatidylinositol 4,5-Diphosphate ; Phosphatidylinositol Phosphates/metabolism ; Phospholipase D/metabolism ; Progesterone/*pharmacology ; Receptors, GABA/physiology ; Spermatozoa/drug effects/*physiology ; Zona Pellucida/*physiology ; gamma-Aminobutyric Acid/pharmacology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 5
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    Generation of functional ventricular heart muscle from mouse ventricular progenitor cells (2009)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2009-10-17
    Beschreibung: The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895998/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895998/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Domian, Ibrahim J -- Chiravuri, Murali -- van der Meer, Peter -- Feinberg, Adam W -- Shi, Xi -- Shao, Ying -- Wu, Sean M -- Parker, Kevin Kit -- Chien, Kenneth R -- K08 HL081086/HL/NHLBI NIH HHS/ -- K08 HL081086-01/HL/NHLBI NIH HHS/ -- K08 HL091209/HL/NHLBI NIH HHS/ -- R01 HL079126/HL/NHLBI NIH HHS/ -- R01 HL079126-01A1/HL/NHLBI NIH HHS/ -- T32 HL002807/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2009 Oct 16;326(5951):426-9. doi: 10.1126/science.1177350.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cardiovascular Research Center, Massachusetts General Hospital, Charles River Plaza, CPZN 3200, 185 Cambridge Street, Boston, MA 02114-2790, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19833966" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Action Potentials ; Animals ; Cell Cycle ; Cell Differentiation ; Cell Line ; Cell Lineage ; Cells, Cultured ; Embryonic Stem Cells/*cytology/physiology ; Gene Expression ; Heart/embryology ; Heart Ventricles/*cytology/embryology ; Mice ; Mice, Transgenic ; Muscle Development ; Myocardial Contraction ; Myocytes, Cardiac/*cytology/physiology ; Oligonucleotide Array Sequence Analysis ; *Tissue Engineering ; *Ventricular Function
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
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    Comment on "Experimental test of self-shielding in vacuum ultraviolet photodissociation of CO" (2009)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2009-06-23
    Beschreibung: Chakraborty et al. (Reports, 5 September 2008, p. 1328) concluded that an anomalously enriched atomic oxygen reservoir can be generated through carbon monoxide photodissociation without self-shielding. We show that this conclusion is based on the incorrect assumption that the spectral shifts of the 97.03-nanometers and 107.61-nanometers vibrational bands for C16O, C17O, and C18O are negligible and point out shortcomings of the low-resolution light source used in their experiments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yin, Qing-Zhu -- Shi, Xiaoyu -- Chang, Chao -- Ng, Cheuk-Yiu -- New York, N.Y. -- Science. 2009 Jun 19;324(5934):1516; author reply 1516. doi: 10.1126/science.1170488.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Geology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. qyin@ucdavis.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19541978" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 7
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    A histone acetyltransferase regulates active DNA demethylation in Arabidopsis (2012)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2012-06-16
    Beschreibung: Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575687/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575687/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Qian, Weiqiang -- Miki, Daisuke -- Zhang, Heng -- Liu, Yunhua -- Zhang, Xi -- Tang, Kai -- Kan, Yunchao -- La, Honggui -- Li, Xiaojie -- Li, Shaofang -- Zhu, Xiaohong -- Shi, Xiaobing -- Zhang, Kangling -- Pontes, Olga -- Chen, Xuemei -- Liu, Renyi -- Gong, Zhizhong -- Zhu, Jian-Kang -- R01 GM059138/GM/NIGMS NIH HHS/ -- R01 GM070795/GM/NIGMS NIH HHS/ -- R01GM059138/GM/NIGMS NIH HHS/ -- R01GM070795/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2012 Jun 15;336(6087):1445-8. doi: 10.1126/science.1219416.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Shanghai Center for Plant Stress Biology and Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22700931" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acetylation ; Arabidopsis/*genetics/*metabolism ; Arabidopsis Proteins/chemistry/genetics/*metabolism ; Chromatin/metabolism ; DNA Glycosylases/metabolism ; *DNA Methylation ; DNA, Plant/*metabolism ; Gene Silencing ; Genes, Plant ; Histone Acetyltransferases/chemistry/genetics/*metabolism ; Histones/metabolism ; Methylation ; Mutation ; Nuclear Proteins/genetics/metabolism ; Protein Structure, Tertiary ; Transgenes
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 8
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    A transforming metal nanocomposite with large elastic strain, low modulus, and high strength (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2013-03-09
    Beschreibung: Freestanding nanowires have ultrahigh elastic strain limits (4 to 7%) and yield strengths, but exploiting their intrinsic mechanical properties in bulk composites has proven to be difficult. We exploited the intrinsic mechanical properties of nanowires in a phase-transforming matrix based on the concept of elastic and transformation strain matching. By engineering the microstructure and residual stress to couple the true elasticity of Nb nanowires with the pseudoelasticity of a NiTi shape-memory alloy, we developed an in situ composite that possesses a large quasi-linear elastic strain of over 6%, a low Young's modulus of ~28 gigapascals, and a high yield strength of ~1.65 gigapascals. Our elastic strain-matching approach allows the exceptional mechanical properties of nanowires to be exploited in bulk materials.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hao, Shijie -- Cui, Lishan -- Jiang, Daqiang -- Han, Xiaodong -- Ren, Yang -- Jiang, Jiang -- Liu, Yinong -- Liu, Zhenyang -- Mao, Shengcheng -- Wang, Yandong -- Li, Yan -- Ren, Xiaobing -- Ding, Xiangdong -- Wang, Shan -- Yu, Cun -- Shi, Xiaobin -- Du, Minshu -- Yang, Feng -- Zheng, Yanjun -- Zhang, Ze -- Li, Xiaodong -- Brown, Dennis E -- Li, Ju -- New York, N.Y. -- Science. 2013 Mar 8;339(6124):1191-4. doi: 10.1126/science.1228602.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23471404" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 9
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    MAVS, cGAS, and endogenous retroviruses in T-independent B cell responses (2014)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2014-12-20
    Beschreibung: Multivalent molecules with repetitive structures including bacterial capsular polysaccharides and viral capsids elicit antibody responses through B cell receptor (BCR) crosslinking in the absence of T cell help. We report that immunization with these T cell-independent type 2 (TI-2) antigens causes up-regulation of endogenous retrovirus (ERV) RNAs in antigen-specific mouse B cells. These RNAs are detected via a mitochondrial antiviral signaling protein (MAVS)-dependent RNA sensing pathway or reverse-transcribed and detected via the cGAS-cGAMP-STING pathway, triggering a second, sustained wave of signaling that promotes specific immunoglobulin M production. Deficiency of both MAVS and cGAS, or treatment of MAVS-deficient mice with reverse transcriptase inhibitors, dramatically inhibits TI-2 antibody responses. These findings suggest that ERV and two innate sensing pathways that detect them are integral components of the TI-2 B cell signaling apparatus.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391621/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391621/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zeng, Ming -- Hu, Zeping -- Shi, Xiaolei -- Li, Xiaohong -- Zhan, Xiaoming -- Li, Xiao-Dong -- Wang, Jianhui -- Choi, Jin Huk -- Wang, Kuan-wen -- Purrington, Tiana -- Tang, Miao -- Fina, Maggy -- DeBerardinis, Ralph J -- Moresco, Eva Marie Y -- Pedersen, Gabriel -- McInerney, Gerald M -- Karlsson Hedestam, Gunilla B -- Chen, Zhijian J -- Beutler, Bruce -- P01 AI070167/AI/NIAID NIH HHS/ -- R01 AI093967/AI/NIAID NIH HHS/ -- R01 CA157996/CA/NCI NIH HHS/ -- U19 AI100627/AI/NIAID NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2014 Dec 19;346(6216):1486-92. doi: 10.1126/science.346.6216.1486.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for the Genetics of Host Defense, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8502, USA. ; Department of Pediatrics and Children's Medical Center Research Institute, and McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8502, USA. ; Center for the Genetics of Host Defense, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8502, USA. Howard Hughes Medical Institute, Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9148, USA. ; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Nobels vag 16, SE-171 77 Stockholm, Sweden. ; Center for the Genetics of Host Defense, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8502, USA. Bruce.Beutler@UTSouthwestern.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25525240" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adaptor Proteins, Signal Transducing/genetics/*immunology ; Animals ; Antibody Formation ; Antigens, T-Independent/*immunology ; B-Lymphocytes/*immunology ; Cytosol/immunology ; DNA/immunology ; Endogenous Retroviruses/genetics/*immunology ; Lymphocyte Activation ; Membrane Proteins/immunology ; Mice ; Mice, Inbred C57BL ; NF-kappa B/metabolism ; Nucleotides, Cyclic/immunology ; Nucleotidyltransferases/genetics/*immunology ; RNA, Viral/genetics/*immunology ; Transcription, Genetic
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 10
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    Genome-scale CRISPR-Cas9 knockout screening in human cells (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2013-12-18
    Beschreibung: The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic repeats)-associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells. First, we used the GeCKO library to identify genes essential for cell viability in cancer and pluripotent stem cells. Next, in a melanoma model, we screened for genes whose loss is involved in resistance to vemurafenib, a therapeutic RAF inhibitor. Our highest-ranking candidates include previously validated genes NF1 and MED12, as well as novel hits NF2, CUL3, TADA2B, and TADA1. We observe a high level of consistency between independent guide RNAs targeting the same gene and a high rate of hit confirmation, demonstrating the promise of genome-scale screening with Cas9.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089965/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089965/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shalem, Ophir -- Sanjana, Neville E -- Hartenian, Ella -- Shi, Xi -- Scott, David A -- Mikkelsen, Tarjei S -- Heckl, Dirk -- Ebert, Benjamin L -- Root, David E -- Doench, John G -- Zhang, Feng -- 1DP1-MH100706/DP/NCCDPHP CDC HHS/ -- 1R01-DK097768/DK/NIDDK NIH HHS/ -- DP1 MH100706/MH/NIMH NIH HHS/ -- R01 DK097768/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24336571" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adaptor Proteins, Signal Transducing/genetics ; Caspase 9/*genetics ; Cell Survival/*genetics ; *Clustered Regularly Interspaced Short Palindromic Repeats ; Cullin Proteins/genetics ; Drug Resistance, Neoplasm/*genetics ; Gene Knockout Techniques ; Gene Library ; Genes, Neurofibromatosis 1 ; Genes, Neurofibromatosis 2 ; Genetic Loci ; Genetic Testing/*methods ; Genome-Wide Association Study ; Humans ; Indoles/therapeutic use ; Lentivirus ; Mediator Complex/genetics ; Melanoma/drug therapy/*genetics ; Pluripotent Stem Cells/*metabolism ; Protein Kinase Inhibitors/therapeutic use ; Selection, Genetic ; Sulfonamides/therapeutic use ; Transcription Factors/genetics ; raf Kinases/antagonists & inhibitors
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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