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  • 1930-1934  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Cytoplasmic structures in binucleate opalinids, with special reference to the Golgi apparatusThis investigation was carried out with the aid of the Cancer Research and Treatment Fund. (1931)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 52 (1931), S. 27-45 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Three types of cell inclusions are demonstrated within the general protoplasm of the binucleated Protoplina, parasites of Hyla aurea. These are considered to represent mitochondria, together with associated, synthesized vegetative granules and Golgi bodies, as evidenced by their behavior, morphology, and staining reactions.The Golgi material is shown to consist of irregularly twisted rods and granules scattered at random in the cytoplasm, but possessing a very distinctive morphology and reaction to different techniques when compared with the mitochondria. No relationship could be detected between these vegetative structures and the cilia, as has been previously described.These observations have also been extended to a similar study of the cytoplasmic organs of Nyctotherus cordiformis, and it has been possible to demonstrate Golgi bodies of a similar appearance within this organism as well as to show again the nature of the basal granules and their relationship to the cilia.The procedure of identifying the mitochondria elements with great care is recommended as a preliminary means of studying the Golgi apparatus of Protozoa, particularly where osmication techniques are used exclusively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050520103
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative cytochemical studies by micro-incineration of a saprozoic and an holozoic infusorianAided by an appropriation from a grant made by the Rockefeller Foundation to Washington University for research in science. (1933)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 54 (1933), S. 389-397 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Nyctotherus cordiformis and Opalinids infesting Rana pipiens were studied by microincineration and in stained control sections. A marked difference in concentration and distribution of mineral salts was noted within saprozoic and holozoic organisms. The endoplasm in the saprozoic protozoa was free from inorganic residue except for the ‘vegetative’ granules; the protoplasm of the holozoic infusorians consisted of a fine network of mineral salts. Detailed examinations suggested that the qualitative and quantitative differences might be correlated with diffusion of digestible material through the cuticle. The inorganic residue of the walls of vacuoles was relatively rich in iron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050540208
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  • 3
    Electronic Resource
    Electronic Resource
    The structure of opalinids, as revealed by the technique of micro-incinerationAided by an appropriation from a grant made by the Rockefeller Foundation to Washington University for research in science. (1932)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 53 (1932), S. 381-388 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Opalinids from the rectum of Rana pipiens were isolated in large numbers and fixed in a mixture of nine parts of absolute alcohol and one part of neutral formalin. These were embedded in paraffin and incinerated according to the technique of Policard. Examination of the preparations by dark-field illumination showed that the micro-incinerated protozoa retained their distinctive morphological and cytological characters, for the mineral ash had the same topographical distribution as the cell inclusions in the control stained preparations.The coarse vegetative granules of the cytoplasm were preserved as distinct masses of ash which corresponded exactly to these bodies in the control preparations. The structure of the myonemata and the cilia was perfectly preserved. The chromatin material of the nucleus, more or less abundant, left little or no ash, which is quite in contrast to the findings of Scott with regard to amphibian and mammalian nuclei.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050530209
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