Publication Date:
1979-06-15
Description:
An aqueous eluant containing a chiral copper-proline complex effects the separation of underivatized amino acid enantiomers on an ion-exchange column. The stereoselectivity is ascribed to differences in stability of the diastereomeric amino acid-copper compexes formed in solution. A simple change in the chirality of the eluant reverses the order of the enantiomer elution. For detection and quantification of picomole amounts of amino acids, the eluant is monitored for fluorescence after reaction with o-phthalaldehyde, a reagent insensitive to proline but highly sensitive for amino acids containing a primary amino group.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hare, P E -- Gil-Av, E -- New York, N.Y. -- Science. 1979 Jun 15;204(4398):1226-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/36662" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acids/*isolation & purification
;
Chromatography, Liquid/methods
;
Copper
;
Hydrogen-Ion Concentration
;
Proline
;
Stereoisomerism
;
Temperature
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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