ISSN:
0032-8332
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A micro-culture technique was developed to determine the optimum culture conditions for the mitogen stimulation of lymphocytes fromMacaca mulatta (rhesus) andMacaca fascicularis (cynomolgus). The optimum concentrations of PHA and Con A ranged from 10–50 µg/culture, those of lymphocytes from 1−2×105 cells/culture, and those of serum from 10–20%. Tritiated thymidine was used at a concentration of .04µCi/culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02381459
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