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  • 1
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    Energetics of malate accumulation in the vacuoles of Kalanchoë tubiflora cells (1981)
    Wiley
    Details
    Publication Date: 1981-04-06
    Print ISSN: 0014-5793
    Electronic ISSN: 1873-3468
    Topics: Biology , Chemistry and Pharmacology
    Published by Wiley on behalf of Federation of European Biochemical Societies.
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  • 2
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    Effects of ecotoxicological chemicals on passive plasmalemma permeability in plants (1983)
    Elsevier
    Details
    Publication Date: 1983-08-01
    Print ISSN: 0147-6513
    Electronic ISSN: 1090-2414
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Elsevier
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  • 3
    Electronic Resource
    Electronic Resource
    2H+:1 malate2− stoichiometry during Crassulacean Acid Metabolism is unaffected by lipophilic cations (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 3 (1980), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Lipophilic cations inhibit nocturnal malic acid accumulation in leaf cells of the Crassulacean Acid Metabolism plant Kalanchoë tubiflora. perhaps by interacting directly or indirectly with active malic acid transport into the vacuoles. Lipophilic cations do not affect passive efflux of malic acid from the vacuoles. Membrane potentials are depolarized, oxygen uptake is stimulated by lipophilic cations and there may also be stomatal responses. Thus it is striking that lipophilic cations do not alter the stoichiometry of 2 titratable H : 1 enzymatically-determined malate2− during diurnal malic acid oscillations of Crassulacean Acid Metabolism in Kalanchoë. This suggests that coupling between protons and malate during transport into the vacuole must be tight. Transport as undissociated acid is unlikely because the dissociation equilibrium in the cytoplasm is largely on the side of malate2−. These results appear to suggest an intimate molecular interaction between a proton pump and a presumed malate2− translocator at the tonoplast of leaf cells with Crassulacean Acid Metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11581541
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  • 4
    Electronic Resource
    Electronic Resource
    Water-relation parameters of giant and normal cells of Capsicum annuum pericarp (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 6 (1983), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Measurements of the water-relation parameters of the giant subepidermal cells (volume, V= 0.119 to 1.658 mm3; 〈inlineGraphic alt="dbl vert, bar (under)" extraInfo="nonStandardEntity" href="urn:x-wiley:01407791:PCE545:Vbar" location="dblvertbar.gif"/〉= 0.53±0.35 mm3, SD, n= 23) and the smaller mesocarp parenchyma cells (V= 0.10 to 0.79×10−3 mm3; 〈inlineGraphic alt="dbl vert, bar (under)" extraInfo="nonStandardEntity" href="urn:x-wiley:01407791:PCE545:Vbar" location="dblvertbar.gif"/〉= 0.36±0.27×10−3 mm3, SD, n= 6) of the inner pericarp surface of Capsicum annuum L. were made using the Jülich pressure probe. The volumetric elastic modulus ɛ for the large cells was between 1.5 and 27 MPa for a pressure range of 0.09 to 0.41 MPa. For the small cells ɛ was 0.1 to 0.6 MPa for a pressure range of 0.22 to 0.39 MPa. The turgor pressure P, the half-time of water exchange T1/2, and the hydraulic conductivity Lp were as follows, with SD and number of replicates: large cells, P= 0.27±0.06 MPa (23), T1/2=2.7±2.2 s (46), Lp=5.8±3.7 pm s−1 Pa− (46); small cells, P= 0.33±0.07 MPa (6), T1/2= 33±10s (12), Lp=0.21±0.07 pm s−1 Pa−1 (12). The determination of these basic water-relation parameters is considered as a prerequisite for future ecotoxicological and phytopathological studies. The differences between the large and the small cells are discussed in relation to a desirable biophysical definition of succulence. Further, for the large cells a pressure and volume dependence of ɛ was demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1983.tb01167.x
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  • 5
    Electronic Resource
    Electronic Resource
    Inhibition of the amino-acid co-transport carrier of Lemna gibba by incorporation ofp-fluorophenylalanine (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 55 (1982), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In Lemna gibba L. a 2 h pretreatment with the amino-acid analogue p-fluorophenylalanine p-FPA inhibited subsequent uptake of L-alanine and reduced the transient depolarization of the plasmalemma caused by L-alanine. Uptake of 3–0-methylglucose was less affected. There was no effect of p-fluorophenylalanine on the resting potential or on the fusicoccin- or light-dependent hyperpolarization of the cells. Furthermore, fusicoccin-stimulation of uptake of L-alanine and 3–0-methylglucose was also unaffected. The results suggest that p-FPA pretreatment selectively acts on the H+-amino-acid co-transport carrier, that the H+-hexose co-transport carrier is much less affected, and that the proton pump appears to be unaffected by p-FPA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1982.tb02293.x
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  • 6
    Electronic Resource
    Electronic Resource
    Uptake of neutral and acidic amino acids by Lemna gibba correlated with the H+-electrochemical gradient at the plasmalemma (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 55 (1982), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Uptake rates of L-alanine, L-serine and L-aspartate and trans-membrane electrical potentials (Δψ) were determined for a pH range in the external medium between 3.5 and 9.0. The proton electrochemical gradients (〈inlineGraphic alt="inline image" href="urn:x-wiley:00319317:PPL351:PPL_351_mu1" location="equation/PPL_351_mu1.gif" extraInfo="missing"/〉) were calculated from Δψ, pH of the medium, and an assumed cytoplasmic pH of 7.5. At external amino-acid concentrations of 0.1 mol m−3, where carrier-mediated uptake dominates total uptake, a linear correlation between uptake rates and 〈inlineGraphic alt="inline image" href="urn:x-wiley:00319317:PPL351:PPL_351_mu1" location="equation/PPL_351_mu1.gif" extraInfo="missing"/〉 is obtained, which extrapolates to zero uptake at zero 〈inlineGraphic alt="inline image" href="urn:x-wiley:00319317:PPL351:PPL_351_mu1" location="equation/PPL_351_mu1.gif" extraInfo="missing"/〉. This corroborates the contention that neutral and acidic amino acids are taken up by Lemna gibba L. by H+-cotransport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1982.tb00304.x
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  • 7
    Electronic Resource
    Electronic Resource
    Estimation of cellular parameters in the compartmental analysis of Li+ transport in Lemna gibba using the stable isotopes, 6Li and 7Li, as tracers (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 62 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using the conventional methods of compartmental analysis has led us to recognize three main compartments in the Lemna cells: the cell-wall, cytoplasm and vacuolc. The half-times of Li+ isotopic exchange were of the order of a few minutes, a few hours and a few days, respectively. The possible existence of a 4th, small, and almost non-exchangeable compartment cannot be excluded. Despite different possible sources of uncertainty (ambiguity of the kinetic solutions, no uniformity of cell types in the plant material, uncertainties in the estimations of electric potentials), the data were consistent with i) Li+ being actively extruded from the cytoplasm, both towards the exterior and the vacuole, ii) the electrochemical potential difference of Li+ being of the order of -25 kj mol across the plasmalemma, and in the range of 5 to 10 kJ mol across the tonoplast, and iii) the passive permeability coefficients of Li+ being in the range of 10 to 10 m s both for the plasmalemma and the tonoplast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb05919.x
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  • 8
    Electronic Resource
    Electronic Resource
    Light-dependent net CO-evolution by C3 and C4 plants (1980)
    Springer
    Planta 149 (1980), S. 59-63 
    Details
    ISSN: 1432-2048
    Keywords: Carbon monoxide ; Crassulacean acid metabolism ; Photosynthesis (C3, C4) ; Photorespiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light-dependent CO-evolution by the green leaves of C3 and C4 plants depends on the CO2/O2 ratio in the ambient atmosphere. This and other physiological responses suggest that CO-evolution is a byproduct of photorespiration. At CO2/O2 ratios up to 10-3, the ratio of CO evolved: CO2 fixed in photosynthesis is significantly higher in C3 than in C4 plants. This discrepancy disappears when a correction is made for the CO2-concentrating mechanism in C4 photosynthesis, by which CO2-concentration at the site of ribulose-bis-phosphate carboxylase/oxygenase in the bundle sheaths is raised significantly as compared to the ambient atmosphere. Since the oxygenase function of this enzyme is responsible for glycolate synthesis, i.e., the substrate of photorespiration, this result seems to support the conclusion that CO-evolution is a consequence of photorespiration. CO-evolution may turn out to be a useful and rather straightforward indicator for photorespiration in ecophysiological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386228
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  • 9
    Electronic Resource
    Electronic Resource
    pH and membrane-potential changes during glucose uptake inLemna gibba G1 and their response to light (1980)
    Springer
    Planta 149 (1980), S. 321-326 
    Details
    ISSN: 1432-2048
    Keywords: Glucose cotransport ; H+-glucose cotransport ; Lemna ; Membrane potential ; pH changes, external ; Transport, glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracellular pH was measured with a microelectrode positioned over the lower surface of singleLemna gibba plants. Upon addition of glucose, a transient extracellular alkalinization occurred. Saturated extracellular pH changes were observed with 5 mM glucose. Simultaneously, the membrane potential difference of −250 mV in the dark measured with intracellular glass micropipettes, trnasiently decreased by 105 mV. Uptake of [14C]glucose and extracellular alkalinization was enhanced by light whereas glucose-induced membrane-potential changes were reduced in the light and became even smaller with increasing the preillumination time. Glucose uptake was optimal at pH 6. The results are taken as further evidence in favor of H+-glucose cotransport inLemna.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571164
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  • 10
    Electronic Resource
    Electronic Resource
    Amino acid uptake by Lemna gibba by a mechanism with affinity to neutral L-and D-amino acids (1980)
    Springer
    Planta 150 (1980), S. 230-235 
    Details
    ISSN: 1432-2048
    Keywords: Amino acids ; Electric membrane potentials ; Lemna ; Membrane transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Earlier work suggested that amino acid uptake by Lemna gibba cells is a H+-cotransport mechanism driven by a proton-electrochemical gradient at the plasmalemma. The present investigations of the transient membrane depolarizations elicited by amino acids and tracer-uptake experiments show that all neutral α-L-amino acids, D-alanine and analogues, like β-alanine and p-fluorophenylalanine, are transported by the same system. It remains to be seen if there are separate mechanisms for the uptake of acidic and basic amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390831
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